血浆胱抑素C在慢性肾功能衰竭中临床应用.docVIP

血浆胱抑素C在慢性肾功能衰竭中临床应用【摘要】 目的 测定慢性肾功能衰竭患者血浆胱抑素C (Cyctalin C)的水平并探讨其临床意义。方法 用免疫比浊法测定l5例肾病综合征，20例慢性肾功能衰竭患者的血浆胱抑素C，其中6例慢性肾功能衰竭患者经肾移植好转后再次测定，另外测定了15例血浆肌酐(Cr)含量正常的急性肾小球肾炎患者血浆胱抑素C含量。并以20例体检正常者作对照。结果 肾病综合征和慢性肾衰竭患者的血浆胱抑素C含量显著高于正常对照组(P0.05)。结论 胱抑素C有助于肾小球滤过功能的评估，血浆胱抑素C是肾脏病患者肾小球损害程度判断和疗效观察有价值的检测指标 【关键词】 胱抑素C;慢性肾功能衰竭;免疫比浊法 Plasma cystatin C with chronic renal failure in clinical application CUI Fan,SHAO Xiao,min.Department of Clinical Laboratory,The First Peoples Hospital of Wuhu city,Wuhu 241000,China 【Abstract】 Objective Determine the cystatin C concentration of plasma from patients with chronic renal failure, and investigate its clinical significance. Methods Cystatin C concentration of plasma samples froml5 patients with nephrotic syndrome, 20 patients (six of them were choused again after being treated with renal transplantation and the condition had turned better)with chronic renal failure and 15 patients with acute glomerulonephritis and normal plasma creatinine (Cr) were detected with immunoturbidimetric. Furthermore, 20 cases from healthy people were taken as the control group. Results Patients with nephrotic syndrome and chronic renal failure, plasma cystatin C was significantly higher than the control (P0.05) between acute nephritis group and the control. Conclusion Cystatin C contribute to the assessment of glomerular filtration function, and plasma cystatin C determine the extent of glomerular damage with kidney disease patients and efficacy testing of value indicators. 【Key words】 Cystatin C;Chronic renal failure;Immunoturbidimetric assay 胱抑素C是一种低分子量、碱性非糖蛋白质，是半胱胺酸蛋白酶的抑制剂，由120个氨基酸组成，分子量为130KDa。可由机体所有有核细胞产生。胱抑素C经肾小球自由滤过，在近曲小管被重新吸收并降解，肾脏是清除循环中胱抑素C的唯一器官。所以血浆胱抑素C浓度主要由肾小球滤过率(GFR)决定，是一种反映GFR变化的理想内源性标志物。由于目前临床上肾小球滤过功能试验存在诸多不足，人们一直在寻找新的可靠便捷反映GFR变化的指标［1］。有研究表明，胱抑素C产生率稳定，血浆浓度受年龄、性别、炎症等影响较小。与GFR的相关性优于血浆肌酐浓度［2，3］。本文检测了50例肾脏病患者血浆胱抑素C的浓度，旨在探讨其临床价值 1 材料与方法 1.1 材料 1.1.1 血浆标本 1.1.1.1 急性肾小球肾炎15例者为我院2010年8月至2011年5月的住院患者。男9例，女6例，年龄范围10~48岁 1.1.1.2 肾病综合征及慢性肾功能衰竭