虫草制品中腺苷和虫草素含量的RP-HPLC分析.pdfVIP

天然产物研究与开发 Nat Prod Res Dev，2007，19：256-259 文章编号：1001-6880{2007)Suppl-0256-04 虫草制品中腺苷和虫草素含量的RP—HPLC分析 邢增涛 ，李光平h，孟范贵2，谢文明2，门殿英 l上海市农业科学院农产品质量标准与检测技术研究所，农业部食用菌产品质量监督检验测试中心 (上海)，上海201106； 吉林农业大学资源与环境学院，长春130118 摘 要：采用超声提取，反相高效液相色谱方法分析了各种虫草制品中的腺苷和虫草素的含量，以含0．05 mol／ L的磷酸二氢钾：甲醇(85／15，V／V)作为流动相。检测波长为260 nnl。进样量为10 ，4o 条件下检测。在5 mg／L到250 mg／L的浓度范围内呈线性相关。研究显示利用蒸馏水在60 超声提取20 wan，腺苷和虫草素的 回收率在85％～95％之间。 关键词：蛹虫草；腺苷；虫草素；反相高效液相色谱 中图分类号：R284．2 文献标识码：A Determination of Adenosine and Cordyeepin in Cordyceps Products by RP-HPLC XING Zeng—tao ，LI Guang—ping ’，MENG Fan—gui ，XIE Wen—ming ，MEN Dian—ying InstituteforAgri-food Standards and Testing Technology ofShanghai Academy ofAgricultural Sciences，Supervision and Testing Centerfor Edible Fungi Q∞Z ，Ministry ofAgriculture(Shanghai)， Shanghai 201106，China； College ofResources and Environmental Sciences， JilinAgriculturul University，Changchun130118，China Abstract：A new RP．HPIE method has been developed for the simultaneous determination of 2 main nucleosides ingre． clients，including adenosine and eordyeepin in Cordyceps．Determination Was achieved on a Diamonsil C18 analytical c01一 unln(4．6 X 250姗 id，5岬 )using methanol water mobile phase elution witll uV detection．Both calibration curves showed good linearity( 0．9995)within the test ranges from 5 to 250 mg／L．-I1le developed method was simple， rapid and accurate，and showed good reproducibility for the quantification of 2 nueleosides in natural and cultured Cordyceps products with an inter—day variations ofless than 3．83％ and 2．28％ for adenosine and eordycepin，respec— tively． Key words：Cordyceps products；adenosine；eordyeepin；RP—HPLC