蜈蚣衣内生真菌研究.pdfVIP

第5卷第4期 菌 物 研 究 V0J．5 No．4 2007年 l2月 Journal of Fungal Research Dec．200r7 Endophytic Fungi Associated with Lichen Physc／a stellaris Using Different Surface Sterilization Methods LI Wen—chao 一，GUO Shou—yu ，GUO Liang—dong (1．Systematic Mycology and Lichenology Laboratory，Institute of Microbiology，Chinese Academy of Sci— enc ，Beijing 100101，China；2．Graduate School of Chinese Academy of Sciences，Beijing 100049， China) Abstract：In a survey of endophytic fungi associated with lichen Physcia stellaris at Dongling mountain in Beijing，six surface sterilization procedures were evaluated to identify the most suitable one for isolating the internal mycobiota of lichen．Various colonization rates(55．4％一100％)of fungi were obtained in the six procedures．A total of 28 fungal taxa were recovered from 316 thallus segments of the lichen．Of these fungi，1 belonged to Ascomycetes，22 to Hyphomycetes，3 to Coelomycetes，1 to Mucor，’and 1 to yeast．The surface sterilization procedure IV(75％ ethanol 1 min，2％sodium hypochlorite 3 min，75％ ethaJ10l 30 S)，as a suitable method for lichen endophyte study，Was selected，based on the analyses of colonization rates and numbers of endophytic fungi obtained in the present study． Key words：Physcia stellaris；endophytic fungus；colonization rate；sterilization procedure CLC nurnl~r：Q949．32 Document code：A Article ID：1672—3538(200-1)04-0202—05 蜈蚣衣内生真菌研究 李文超 2，郭守玉 ，郭良栋 (1．中国科学院微生物研究所真菌地衣系统学重点实验室，北京 100101；2．中国科学院研究 生院，北京 100049) 摘 要：对北京东灵山地衣蜈蚣衣进行内生真菌分离，经过6种不同的方法消毒后，内生真菌的定殖率为 55．4％～100％；从316个地衣组织块上共分离到28个真菌分类单元，其中包括1个子囊菌，22个丝孢菌，3个 腔孢菌，1个毛霉和1个酵母菌。根据定殖率