新型聚乙二醇改性聚乳酸-多烯紫杉醇纳米粒体外 - 第三军医大学学报.docVIP

新型聚乙二醇改性聚乳酸-多烯紫杉醇纳米粒体外抗肿瘤研究 赵俪梅1，张寅星2，陈振波1，梁志清1 （400038重庆，第三军医大学西南医院妇产科1；400044重庆，重庆大学生物工程学院2） [摘要] 目的 以人卵巢癌细胞SKOV3为模型,研究负载多烯紫杉醇（TXT）的新型聚乙二醇改性聚乳酸纳米粒（TXT-PPLA NPs）的体外抗肿瘤作用。 方法 以不同剂量TXT-PPLA NPs（1、10、100、1 000、10 000 nmol/L）处理SKOV3细胞不同时间（12、24、48、72、96、120 h）后用CCK-8测定细胞生长抑制率，流式细胞仪检测细胞凋亡率，Western blot检测Caspase-3表达。 结果 在10～10 000 nmol/L浓度范围内，相同时间点下，TXT和TXT-PPLA NPs对SKOV3细胞生长的抑制率均呈现出剂量依赖性。在相同浓度下，TXT组在24 h和48 h对SKOV3细胞的生长抑制率、凋亡率及capase-3的表达均明显高于TXT-PPLA NPs组（P 0.05）；72 h后，TXT-PPLA NPs组对SKOV3细胞的生长抑制率、凋亡率及capase-3的表达均明显高于TXT组（P0.05 ）。结论 与TXT相比，TXT-PPLA NPs能够更加持续地抑制SKOV3细胞的增殖，并促进caspase-3介导的细胞凋亡，具有更强的持续性抗肿瘤作用。 [关键词] 多烯紫杉醇；聚合物纳米微粒；卵巢癌；细胞增殖；细胞凋亡；caspase-3 [中图法分类号] [文献标志码]A In vitro antitumor efficacy of novel docetaxel-loaded polyethylene glycol-modified poly(lactic acid) nanoparticles Zhao Limei1, Zhang Yinxing2,Chen Zhenbo1, Liang Zhiqing1 (1Department of Gynecology and Obstrics, Southwestern Hospital, Third Military Medical University, Chongqing, 400038; 2College of Bioengineering, Chongqing University, Chongqing, 400044, China) [Abstract] Objective The study aims at exploring antitumor effects of the novel docetaxel (TXT)-loaded polyethylene glycol (PEG)-modified poly lactic acid nanoparticles (TXT-PPLA NPs) on human ovarian cancer cell SKOV3 in vitro. Methods SKOV3 cells were cultured with TXT-PPLA NPs in different concentrations (1,10,100,1000,10000 nmol/L) and durations (12,24,48,72,96,120 h). Then proliferation and apoptosis of SKOV3 cells were determined by CCK-8 assay and flow cytometry, respectively. The apoptosis-related protein caspase-3 was measured by Western blot. Results Both TXT and TXT-PPLA NPs could inhibit the proliferation of SKOV3 cells in a dose-dependent manner between the concentration of 10-10000 nmol/L within the same duration. Proliferation rate, apoptosis rate and expression of caspase-3 of TXT SKOV3-treated cells were obviously higher than those of TXT-PPLA NPs-treated group in the same concentration after both 24h and 48h;