产聚羟基丁酸酯菌株的筛选及发酵条件的优化.PDF

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 10 1 崔志芳 季爱云 李春露 (, 266510) : 作为一类可望替代传统塑料的新型可降解生物高分子 料, 聚羟基丁酸酯( PH B) 日益引起人们的重视采 用尼罗蓝荧光法从污水中初筛得到产 PHB 的细菌, 摇瓶发酵复筛得到一株 PHB 产量较高的菌株 AE 13, 同时对该菌株的发酵 条件进行了正交优化, PH B的产量达到 085 g/L : 聚羟基丁酸酯 尼罗蓝 筛选 发酵 Screening of Strains for A ccum ulating P olyhydroxybu tyrate and Studying on Its Ferm entation Conditions Cui Zhifang JiA iyun L iChunlu (Th e Colleg e of Chem ica l E ng ineering, S handong Un iversity of S cience and Technology, Q ingdao 2665 10) Abstrac:t A s a n w kind of polym r m at rial xp ct d to r p lac th traditional plastics, polyhydroxybutyrat ( PH B) aros grow ing att ntion in r c nt y ars. In th is pap r, strains producing PHB w r scr n d from th sludg by N il b lu fluor sc nc . A strain w ith h igh PH B cont nt was nam d AE 13. Furth rm or , th optmi ization of f rm ntation condition was stud i d by orthogonal xp rmi nt. Und r th optmi al condition, PHB cont nt of AE 13 could b r ach d 085g /L. K ey w ords: Polyhydroxybu tyrat ( PHB ) N il b lu Scr n ing F rm ntation , B B , , , [ 2] , , , PHB [ 1] , ( polyhydroxyal , kanoat s, PHAs) PHB PHA s , , (), PHB , PHA s 1 150, 11 料 , ( poly 111 hydroxybutyrat , PHB) PHB PHB S igm aA ldrich, Ak aibao, , ,