Validation of Abacus SALIgAE Test for the Forensic Identification of Saliva：（验证Abacus SALIgAE测试唾液的法医鉴定）.pdfVIP

Validation of Abacus SALIgAE® Test for the Forensic Identification of Saliva David W Miller, Jason C. Hodges WV State Police Forensic Laboratory October 7th, 2005 2 Abstract Saliva stains can play an important part in the forensic investigation of violent crimes by ® linking a suspect to DNA from saliva found at a crime scene. The SALIgAE saliva test from Abacus Diagnostics, a colorimetric test, provides a new alternative to the saliva tests of the past. This test is based upon the presence of salivary amylase, a compound present in high concentrations in human saliva. In this study, samples extracted from mock casework samples as well as dilution series were dispensed into the SALIgAE® test vials and measured for color change against a negative control. All samples as well as the dilutions up to 1:1000 tested positive for saliva with varying degree of color change depending on dilution of the sample. In the sensitivity portion of the study it was found that dilutions up to 1:1000 gave results, but dilutions past 1:400 were difficult to read. All of the samples we expected to be positive gave positive test results, except some samples in the blind soda can study and the ski mask study. The success of this test in the mock casework showed that this test has the potential to give positive results on evidence, such as cigarette butts and soda cans, that we regularly receive in our lab. Based on the results from this study the SALIgAE® test has proven to be sensitive, simple, accurate, and could become a valuable tool to our lab. 3 Introduction S