Folin-Ciocalteu法测定苹果醋饮料的总多酚含量.pdfVIP

※王艺技术 画面融事 20日~ VoJ.34, No.08 31 Folin-Ciocalteu法测定苹果醋饮料的总多酣含量 赵晓娟，李敏仪，黄桂颖，陈悦娇 (仲皑农业工程学院轻工食品学院，广东广州 510225) 摘 要z 研究Folin-Ciocalteu法测定苹果醋饮料中总多酌含量的最适测定条件，并对该方法进行了分析评价。结果 表明多盼含量的最佳测定条件为:样品用量1.0mL、福林，盼试剂2.5mL、 12%碳酸纳溶液4.伽nL、反应温度45.C 、 反应时间1.5h、测定波长756nm。吸光度与多盼含量在0~6μglmL范围内呈良好的线性关系。=0. 1238x +0.OO98, 2 R =O.9992) ， 方法的加标回收率为101.2%，相对标准偏差为2.8%(n=匀。该方法精密度高、稳定性好，适用于苹果醋 饮料中总多盼含量的测定。 关键词: Fo1in-Ci侃a1teu; 分光光度法:苹果醋:多酣 Determination of Total Polyphenols in Apple Vinegar Drink by Folin-Ciocalteu Me也od ZHAO Xia。如阻， LI Min-yi , HUANG Gui-yi吨， CHEN Yue-jiao (College ofLight Industry and Food Science, Zhongkai University of Agricu1ture and Engineering, Guangzhou 510225 , China) Abstract: The optimum condition on determining total polyphenols content in apple vinegar drink by Folin-Ciocalteu method was studied. The resu1ts indicated that the optimum conditions for tota1 po1yphenols determination by Folin- Cioca1teu were as follows: 2.5 mL of Folin-phenol reagent was added in 1.0 mL of s缸nple， and then 4.0 mL of 12% sodiurn carbonate solution was added. After the coloration reaction was for 1.5 h at 45 C, the optica1 density was measured at 756 nm. Under the optimum conditions, the absorbance increased linearly with the concentration of ga1 1ic acid in the range of 2 oto 6μglmL (y=0.1238x+0.OO98 , R =0.9992). The average spiked recovery rate was 101.2% with 出e relativ巳 standard deviations of 2.8% (n=5). τhe method is highly precise and stable, and suitable for determining tota1 polyphenol content in apple vinegar drink. Key words: Fo1in-Cioca1teu; spectrophotometry; apple vinegar; polyphenol 中图分类号: TS207 .3 文献标志码:A 文章编号: 1∞2-6630(2013)08-∞31-05