GC-MSMS测定镇江香醋中的氨基甲酸乙酯.pdfVIP

2016 年第35 卷第8 期 中国酿造 总第 294 期 .159- 分析与检测 GC-MSIMS测定镇江香醋中的氨基甲酸乙醋 唐双双，王韦岗 (镇江市产品质量监督检验中心，江苏镇江 212132) 摘 要:镇江香醋中的氨基甲酸乙酶(EC)通过乙酸乙醋提取，用N-丙基乙二肢(PSA)分散固相萃取的方法净化，采用气相色谱.串联质谱 (GC-MSIMS)法测定。结果表明，氨基甲酸乙酶(EC)的回收率达到96.7%，标准曲线的相关系数贬为0.9997 ，最低检出限为lμglkgo 该方法简单、快速、灵敏，能有效减少杂质干扰。测定二十份不同厂家生产的镇江香醋，均检出氨基甲酸乙酶CEC) 。表明镇江香醋在 生产发酵过程中，不可避免的会发生EC污染，应采取措施进行控制。 关键询:镇江香醋:氨基甲酸乙酶:气相色谱.串联质谱:分散固相萃驭 中图分类号:TS264 文章编号:0254-5071 (2016) 08-0159-04 doi:10.1188剖.issn.0254-5071.2016.08.036 Determination of ethyl c的amate in Zhenjiang vinegar by GC-MSIMS TANG Shuangshuang, WANG Weigang (Zhe吨， ;iang Institute ofSupervision and Inspection ofProduct Q.国1ity， Zhenjiang 212132, China) Abstract: Ethy1 carbamate (EC) in zh叫iang vineg缸was extracted by ethy1 acetate，也e solution was purified by prim町secondary amine dispersion solid-phase extraction me也od， then was detennined by GC-MSIMS. The results showed 也at 也e re币overy rate ofEC was up to 96.7%, correlation co- 2 efficient R of sta且也rd curve was 0.9997，也.e lowest limit of detection was 1 件glkg. The method was simple, fast and sensitive, which cou1d effec- tively reduce the interference of impurities. EC was detected in 20 Zhenjiang 功negar products 企om different manufacturers , which indicated 出at EC pollution was unavoidable during the process of Zhenjiang vinegar fennentation , and measures should be taken to control. Key words: Zhenjiang vinegar; ethyl carbamate; GC-MSIMS; dispersive solid phase extraction 氨基甲酸乙醋Cethyl carbamate ,EC) 是发酵食物和酒 光检测法Chigh performance liquid chromatography-fluores- 精饮品在发酵和贮存过程中天然产生的污染物，在发酵食 ,HPLC-FLD) [lO] 。液相色