HPLC法测定苹果酸阿莫曲坦含量的研究.pdfVIP

安徽农业科学，Joumal of Anhui A伊. Sci. 2015 ,43 (的 :7 -8 责任编辑刘月娼责任校对况冷玲 HPLC 法测定苹果酸阿莫曲坦含量的研究 周唯兰，吴品江，周芯宇，颜欢，李元波 (扬子江药业集团四川海蓉药业有限公司，四川成都 611830) 摘要 [目的]建立一种高效液相色谱法，测定苹果酸阿莫曲坦片的含量。[方法]采用高效液相色谱法(HPLC) ，色谱柱为 Agílent Zobax S8 c8 column (250 x4.6 nun ,5 f.UIl );以缓冲液(取0.5 g 辛炕磺酸纳与5ml 磷酸溶液，加水至 1α)() ml ，用 5 mo.νL 氮氧化纳调节pH 至 3.0) -乙蜻作为流动相，等皮洗脱，流速1.0 mνmm，拉温 30 t，检测波长 230 nm，进样量 10μ。[结果]苹果酸阿莫曲坦的浓度在 17.49 斗的.88μglml 范围内线性关系良好(Y =27. 534X +9. 693 ,r =0.999 9) ，高、中、低 3 种浓度的平均回收率(n =3) 为 99.23% (RSD =0.957毛) ;中间精密度RSD =0. 98% (n =6) ，重复性和耐用性良好。[结论]该方法简便、灵敏、准确，可作为苹果酸阿莫曲坦片含量的 检验方法。 关键词 苹果政阿莫曲远;含量源IJ 定;HPLC 申图分类号 5854.5 丁 文献标识码 A 文章编号 0517 -6611(2015)08-即7-但 Determ画aüon of Almotriptan Malate Tablets by HPLC ZHOU Wei-Ian, WU pin寸iang ， ZHOU Xin-yu et aI (Yangtze River Pharmaceutical Group 5ichuan Eron Pharmaceutical Company Lim- ited , Chengdu , 5ichuan 611830) Abstract [ Objecive] To es组blish a HPLC me也d for the determination of almotriptan malate tablets. [MethodJ Using high-performance liquid chromatography , chromatographic column of Agilent zobax 58 C8 column (250 x 4. 6 mm , 5μm) ; With a mixture of octane sulfonate buffer (adjusted pH to 3.0 wi出 phosphoric acid) and acetonitrile 田 mobile phase , isocratic elution method , flow rate 1. 0 mνmin ， tempera- ture 300 C , detection wavelength 230 nm , injection 10 tJl. [Result] Almotriptan malate concentration in the range of 17. 49ω2佣.88 忡喜Iml h国 a good linear relationship (Y = 27.534X + 9.693 , r = 0.9999) ，出e average recovery of high , middle. low , 3 kinds of concentration ratio ( n =3 ) 99.23% (RSD =0. 95%); intermediate precision RSD =0.987毛( n = 6) , Repeatability and durability is good. [Conclusion J ηle method is simple , sensitive , accurate , reproducible , which is suitable for the determination of almotriptan malate Key words Almotriptan malate; Content determínation; HPL