HPLC测定银线草中酚酸的含量.pdfVIP

安徽农业科学，Journal of Anhui Agri. Sci. 20. 15 ,43 (31) :74 -76 , 100 责任编辑黄小燕责任校对况冷玲 HPLC 测定银线草中酣酸的含量 ~王王王1: 1 ,2 恭今今 (1.荷泽学院生命科学系，山东荷泽 274∞0. ;2. 陕西师范大学药用资源与天然药物化学教育部重点实验室，西北濒危药材资源开 发国家工程实验室，陕西西安 710062) 摘要 [目的]采用 HPLC 方法测定不同部位不同采收季节银线草中盼酸类含量。[方法]采用高效液相色谱法，色谱柱为 Phenornenex C18 (250. rnrn x 4.6 rnrn ， 5 件rn) ;流动相甲醇-0..1% 甲酸线性梯度洗脱，流速为0..8 rnνmin;检测l 波长323 nrn ，柱温25{;o[ 结果]银线草 中迷迭香酸、反丁烯二酸、绿原酸在测定范围内线性关系较好(r:;;: o. .999 1) ，平均回收率为 97.5% -105.1%; 不同部位不同采收季节银 线苹盼酸类含量存在一定差异。[结论]该方法操作简便、定量准确、重现性好，可用于银线草中国命酸类含量的测定。 关键词银线苹;HPLC; 盼酸;含量测定 中图分类号 S567. 23 +9 文献标识码 A 文章编号0.517 -6611(20. 15)31-074-03 Detennination of the Phenolic Acids Content in Chloranthus j，吻。nicus Sieb. by HPLC YUAN Qin_qinl.Z (1. Departrnent of Li岛Science ， Heze University , Heze ,Shandong 274000 ;2. Key Laboratory of Medicinal Resource and Nat- ural Pharrnaceutical Chernistry of Ministry of Education ,National Engineering LaboratOIγfor Resource Developing of Endangered Chinese Crude Drug in Northwest of China , Shaanxi Norrnal University , Xi an ,Shaanxi 71仪)62) Abstract [ ObjectiveJ Deterrnination of different parts and seasons of phenolic acids content in Chloranthus jα， rponicus Sieb. by HPLC. [Meth- ods) The 巾。rnat哺aphic 叫arations we陀 obtained by the Phenornenex C18(250. rnrn x 4. 6 rnrn ,5 川)with 出e gradient elution solvent 酬ern cornposed of rnethanol and 0.. 1 % forrnicacid ,the flow rate was o.. 8 rnνrnin ， the wavelength was set at 323 nrn and the colurnn ternperat盯e was rnaintained at 25 {;. [阳Resu山1 cωov刊er巧y between 叨97.5%毛 一 10.臼6.1%毛.咽1回ere w副 a certain diffe岛缸er，陀en配ce of dif俗T元erent p盯t恒s and 肥sea酣so佣ns ofp卢h阳e臼enoli阳c acids content in Chlo曰ra阻nt由hus 归j ap仰omcus S 缸ieh. . [Conclusion] The me由odw田 simple ， acc町ate quantitative and good reproducihility ,which can he used for the deterrnination of phenolic acids content in Chloranthusjaponicus