HPLC法测定奈米非肽中三氟醋酸含量.pdfVIP

军军进修学院学报 1 Chínese PLA P08t呈rad Medsch Apr2011 , 32{4) .349. HPLC 法测定奈米非肢中三氟醋酸含量 肆宣鸿 1 ，李秀教2 1 解放军总墨院医学保障部，北京 100853 ; 2 北京军军总医院药剂巷，北京 1007∞ 摘要:目前建立HPLC 测定奈米非放中三氟醋酸含量的方法。方法色谱柱: Apollo C18(4.6mm x 2给nm， 5 抖叫:流动相: 流动相AO.7% 磷酸水溶液(PH3.0) ，流动相B 甲醇 z 流速: 1.2mVmi延;检测波长: 21 0nm ;进样量 :10μ1。结果在12.0- 961.2μglml 浓度革围内，呈良好线性关系，线性方程为A::O.232 2X-O.062 6 , r=O.999 6，平均国收率 1∞.7%.RSD 为0.73% 伊边}。结论本法简单可靠，重现性好，可作为奈米非放中三氟蜻酸含量翻定方法。 关键词:奈米非肤;三氟醋酸; HPLC; 测定 中图分类号 :R9 文献标识码 :A 文章编号: 1∞5-1l39(却11)04-0349-02 Determination of trißuoroactic acid Jevel in nemifitide by HPLC 1 2 CHEN Yi-hong , LI Xiu-m.in I Division of Medical S饵urity， Chinese PLA General Hospi时， B喝ing 1∞塞到， China; 2Department of Pharmacy, Be.运ing Army General Hospital , Beijing 1∞7∞， Cruna The first autb倍: CHEN Yi-hong. Email: 9578CYH@sÎna .com Abstract: Objective To establish the HPLC method for measurement of trifluoroactic acid level in nemifitide. Methods Chromatogram with 扭Apollo C18ωlumn(4.6mm x 25Omm, 5μ盼， mobile phase with a 0.7% phosphoric acid aqueous solutωn (pH3剧， methanol with a flow vel(町.ty of 12ml1m函， test waveleng也就21Onm， and 姐Íl飞jection dose of 10j.1. 1, were used to establisb 往le HPLC method for n怒asurement of 往ifluor，。在ctic acid level in nemifitide. Results The method had a good 益near rela延on when the 纪gression 呵uation was A::0232 2X-O .062 6 and r=O织)9 6 at 也s ∞瓢:en出主ion of 12.02-961 勾tglml. 在le average l1伐。very rate was 1∞.7% and 也e RSD was 0.739毛位=6). Condusion 白le method is siInple 扭d reliable with a g!α艰re萨现ability. and c姐也:us be g部d in Rleas1lr军ment oftriflu倪。副ic acid level Íll nemifitide. Key words: Nemifitide; Tritluoroactic Acid; HPLC; 1元回mination 奈米非肤(Nemifitide) 是一种人工合成的五肤