HPLC测定11种中药口服液中6种防腐剂的含量.pdfVIP

第 18 卷第 3 期 中国实验方剂学杂志 Vol. I8 ,No.3 2012 年 2 月 Chinese Journal of Experimental Traditional Medical Formulae Feb. ,2012 HPLC 测定 11 种中药口服液中 6 种防腐剂的含量 孙蒙*，闰小玉，毕开j顷，陈晓辉 (沈阳药科大学药学院，沈阳 110016 ) [摘要] 目的:建立 HPLC 同时测定中药口服液体制剂中防腐剂含量的方法。方法:Kromasil C 色谱柱(4.6 mm x 250 1S mm， 5μm) ，流动相乙膀。.02 mol.L 哥斗1 乙酸镀溶液梯度洗脱，流速1. 0 mL.叮ml 山梨酸、对英瓷E基苯甲酸醋类在 l仁.0 町 18创1. 1 mg L- 1 (υr O. 99佣9) ;平均回收率 9归5.5%毛- 10∞0.8%串 (RSD 0.7%串卢 线性关系良好 1. 5%)儿。结论:方法简单、可靠，精确，可用于中药口服液体制剂中防腐剂含量的测定。 [关键词] 口服液体制剂:苯甲酸:山梨酸;对经基苯甲酸酶;jj!tl定 [中图分类号] R284. 1 [文献标识码] A [文章编号] 1005-9903 (2012) 03 -0076 -04 Simultaneous Determination of Preservatives in Traditional Chinese Oral Liquid Preparation by HPLC SUN Meng , YAN Xiao-yu ,BI Kai-shun , CHEN Xiao-hui (School 01 Pharmacy , Shenyα昭 Pharmaceutical Uni阳sity ， Shenya昭 110016 ， Chinα) [ AbstractJ Objective: To establish a HPLC method for simultaneous determination of prßservatives in traditional chinese oral liquid preparation. Method: The chromatographic separation was achieved on a Kromasil CI8 (4. 6 mm x 150 mm , 5μn) column with acetonitrile -O. 02 mol. L -1 ammonium acetate solution as mobile phase (gradient elution). The flow rate was 1. 0 mL min - 1 , the detective wavelength was set at 240 nm , and the column temperature was maintained at 30 C. Result: Benzoic acid , sorbic acid and parahydroxybenzoate ester have a good resolution and linear correlation in certain range , respectively (r 0.999). The average recoverieswere 95.5% - 100. 8 % (RSD 1. 5 % -1. 8 % ). Conclu