人血浆中匹维溴铵的LC-MSMS法测定.pdfVIP

中国医药工业杂志 Chinese Jouma1 of Pharmaceutica1s 20口， 44 (5) • 499 • 人血浆中匹维漠银的 LC-MS/MS 法测定 赵曦l ，2 ，杨汉、应l 川，任进民3 ，靳怡然气吴瑕3 (1.石药集团中奇制药技术(石家庄)有限公司，河北石家庄 050051; 2. 河北省制剂工程技术研究中心，河北石家庄 050051; 3. 河北医科大学第二医院，河北石家庄 050000) 摘要:建立了液相色谱-串联质谱法测定人血浆中的匹维澳馁。以紫杉醇为内标，采用 C 色谱柱，流动相为乙腊- l8 2 mmo1/L 乙酸钱溶液-甲酸 (70 : 30 : 0. 1)，多反应监测 (MRM) 扫描，监测离子对 m/z 512.5 • 23 1.3 (匹维澳饺)和 m/z 854.3 • 286.3 (紫杉醇)。低、中、高浓度匹维澳钱的血浆提取回收率分别为 11 1. 7% 、 104.9%和 99.7% ，日内和日 间 RSD 均小于 15% 。 关键词:匹维澳馁:液相色谱-串联质谱:测定 中图分类号: 0657.7+2 文献标志码:A 文章编号: 1001-8255 (2013) 05-0499-04 Determination ofPinaverium in Human Plasma by LC-MS刷1S l 1 川 3 3 3 ZHAOXi YANGHan严1 ， REN Jinmin , JIN Yiran , WU Xia 气 (1. Pharmaceutical Research Institute ofShijiazhuang Pharmaceutical Group Co. , Ltd., Shijiazhuang 050051; 2. Hebei Pharmaceutical Engineering Technology Research Center, Shijiazhuang 050051; 3. The Second Hospital ofHebei Medical Uniersi吵; Shijiazhuang 050000) ABSTRACT: A LC-MS/MS method was established for the determination of pinaverium in human plasma. Paclitaxel was used as the intemal standard. A C I8 column was used with the mobile phase of acetonitrile-2 mmol/L ammonium acetate-formic acid (70 : 30 : 0.1) in multiple reaction monitoring (MRM) mode with the transitions of m/z 512.5 • 231.3 (pinaverium) and m/z 854.3 • 286.3 (paclitaxel) , respectively. The recoveries of pinaverium at low, medium and high concentration levels were 11 l.7% , 104.9% and 99.7% , respectively. The inter- and intra-day RSDs were