分子印迹-基质固相分散-高效液相色谱法测定血液中苯磺酸氨氯地平.pdfVIP

PTCA(PART B: CHEM. ANAL.) 分子印迹-基质固相分散-高效液相色谱法 测定血液中苯磺酸氨氯地平 许文静，张社利，张文生 (焦作师范高等专科学校分离与吸附材料工程技术研究中心，焦作 454001) 摘要:采用分子印迹-基质固相分散-高效液相色谱法(MI-MSPD-HPLC) 测定血液中痕量苯 磺酸氨氯地平。制备苯磺酸氨氯地平分子印迹聚合物，以此为基质固相分散剂与样品混合均匀，风 干后装柱，用甲醇洗脱苯磺酸氨氯地平。洗脱液在Inertsil DOS-SP 分离柱上分离，以甲醇乙睛水 (70+22+8) 混合液为流动相进行洗脱，检测波长为 237 nmo 氨氯地平的线性范围为1. 7 ~ 20. 0μg·L1 ，检出限(3SjN) 为 O. 5μg.L一1 ，测定下限(lOSjN) 为1. 7μg.L一l 。对空白血液样 品进行加标回收试验，回收率在 9 1. 0%~109%之间，测定值的相对标准偏差(n=5) 在 3. 7%~ 4.8%之间。方法可用于测定血液中的苯磺酸氨氯地平。 关键词:高效液相色谱法;印迹聚合物;基质固相分散;苯磺酸氨氯地平;血液 中固分类号: 0657.7 文献标志码:A 文章编号: 1001-4020(2015)04-0446-04 Determination of Amlodipine Besylate in Blood by HPLC Combined with Molecular Imprinting Matrix Solid Phase Dispersion XU Wen-ji吨， ZHANG She-Ii, ZHANG Wen-sheng (Separation and Adsorption Materials Engineering Technology Resωrch Center , ] iaozuo Teachers College , ] iaozuo 454001 , China) Abstract: A method of HPLC combined with molecular imprinting matrix solid phase dispersion was applied to determine amlodipine besylate in blood. Molecularly imprinted polymer for amlodipine besylate was synthesized and mixed with the sample , used as matrix solid phase dispersing agent. After air-drying , the mixture was used as filter for packing column , and amlodipine besylate was eluted from the column by methano l. The eluent was separated on an Inertsil OOS-SP column usÎng a mÎxture of methanol-acetonitrile-water (70 十 22 +8) as mobile phase. UV absorbance was measured at 237 nm. The linearity range of amlodipine besylate was 1. 7 - 20. 0μg L-) , with detection limit (3S/N) of o. 5μg L-) and lower limit of determination (lOS/N) of 1. 7μg L-). The recovery rates measured by s