口腔颌面部原代鳞状细胞癌药物敏感实验_李雅冬.pdf

2007 2 (27 2 )　Stomatology, Vol.27, No.2, Feb.2007 ·77 · 1 1 1 1 1 2 李雅冬,杨　凯,季　平,张福军, 陈　睿, 陈　勇 [] 　　。　 (SRB), 。　SRB :+ ;。　SRB , 、 , , ;。 [] 　;;; [] 　R916.696　　[]　A　　[] 　1003-9872(2007)02-0077-03 Research of drug sensitivity assay of primary culture of oral maxillofacial squamous cell carcinoma LI Ya-dong, YANGKai, JI P ing, ZHAN G Fu-j un, CHEN Rui, CHEN Yong .(Department of Ora and Max i of acia S urgury, theF irst Aff i i- ated Hosp ita Chongqing University of Medica Sciences, Chongqing 400016, China ) Abstract:　Objective　To seek an effective drug and best drug sensitivity assay to be a lied to clinical medicine.Methods　The tech- nique of rimary culture of squamous cell carcinoma and SRB assay was used to get the result of drug sensitivity assay of oral maxillofacial squa- mons cell carcinoma.Results　The results of drug sensitivity assay were as follows:The order of the average inhibition of all drugswas CBDCA +5-FuCBDCA 5-Fu PYM MTX;the average inhibition of the higher concentrationwas bigger than that of the lower concentra- tion.Conclusions　SRB assay is ra id, chea , sim le and suitable to medical sciences, It meets the clinical requirements and is suitable to o ularize.The effect of combinationwas better than that of the single drug. Key words:　oral cancer;cell culture;drug sensitivity assay;SRB assay Stomatology, 2007, 27(2):77-79 　　() (CBDCA):;(PYM): , , ;(MTX): , ; ;(5-Fu):。 , , :3 , 。 2 (PPC) 、 mg× 100 , PPC(μg/ml)= × (60 60 , kg )。 1、3 PPC 10 。 、1/10。