内皮源性一氧化氮合酶基因多态性与缺血性脑卒中相关性研究.pdfVIP

· 644· J Apoplexy and Nervous Diseases，December 2007，Vol 24，No．6 文章编号：1003—2754(2007)06—0644 内皮源 氧化氮合酶基因多态性与 缺血性脑卒中相关性研究 高 鹏， 吴 江， 杜丹华， 赵节绪， 胡林森， 林文华 摘 要： 目的 通过病例对照研究，探讨内皮源性一氧化氮合酶 (eNOS)基因多态性与缺血性脑卒中的关 系。方法 采用聚合酶链反应(PCR)和限制性片断长度多态性(RFI P)技术，对452例缺血性脑卒中患者和153例 健康对照人群的eNOS基因rs3918181位点进行基因多态性检测。结果 大动脉粥样硬化型脑梗死组的基因型与等 ∞ 位基因频率与正常对照组比较P0．05，无统计学意义 腔隙性脑梗死组eNOS基因AA+AG基因型频率明显高于 对照组．相对于GG基因型，暴露于AA+AG基因型人群的OR值为1．644(95 CI 1．124～2．405)。腔隙性脑梗死 A等位基因频率也显著高于对照组，相对于G等位基因。A等位基因OR值为1．41 9(95％CI 1．061～1．898)。结论 一 内皮源性一氧化氮合酶(e—NOS)基因rs3918181位点多态性与腔隙性脑梗死相关；A等位基因可能增加中国汉 族人罹患腔隙性脑梗死的风险。 关键词： 内皮源性一氧化氮合酶； 脑卒中； 基因多态性 中图分类号：R743．32 文献标识码：A Association study between a polymorphism of endothelial nitric oxide synthase gene and ischemic stroke GAO I~eng，WU ]iang，DU Dan—hua，Pt a1．(Department f)j Neurolog3，，the First Hospital of ]ilia University，Changchun 130021。China) Abstract： Objective To explore the relationship of endothelial nitric oxide synthase(eN()S)gene polymor— phism and ischemic stroke through case—control study．Methods 452 patients with isehemie stroke were enrolled into this study，153 healthy people were as controls．SNP rs3918181 of the gene was used as a genetic marker． Rs3918I81 polymorphism of eNOS gene was monitored by polymerize chain reaction fragment length polymor— phism(PCR—REI P)．Results The tesl showed no association between healthy controls and patients with cere— bral infarction due tO large vessel atherosc1erosis．In patientes with lacunar infarction，the frequency of allele A of rs3918181 was significantly higher than that in controls( 一5．560，P一0．018。OR一1．419，95％CI1．061～ 1．898)．And the frequency of AA and AG genotypes of rs3918181 was significantly higher in pati