class 10第四章 神经生物物理学1.ppt

第四章 神经与感官生物物理;本章授课内容安排;§1 细胞电活动－解释各种生物电、生物磁现象和效应的基础;Outline;K+;膜两侧正负电荷不平衡;;1.1.2 离子平衡电位－理解生物膜的所有电现象的基础;Nernst方程－离子平衡电位的计算;要建立细胞膜上某离子的平衡电位，要求：;Ionic Basis of generation of membrane potential Nernst Equation Goldman-Hodgkin-Katz (GHK) equation Electrical model of cell membrane Electrical excitability and mechanism of generation of action potential Voltage clamp patch clamp technique;§1.2 膜电位;哺乳动物骨骼肌细胞内外离子成分及其浓度、平衡电位;Membrane potential versus extracellular [K+];Three facts we learned from the previous experiments;The Goldman-Hodgkin-Katz voltage equation, also known as the Goldman equation, is used in electrophysiology to determine the equilibrium potential across a cells membrane taking into account all of the ions that are permeant through that membrane. It is established by David E. Goldman, and the English Nobel laureates Alan Lloyd Hodgkin and Bernard Katz.;Goldman-Hodgkin-Katz 方程;细胞膜对K+、Na+不同通透性对静息电位产生的影响;细胞膜对K+、Na+不同通透性对静息电位产生的影响;The resting membrane potential is determined by resting ion channel Resting channels in Glial cells are selective for potassium only Resting channels in nerve cells are selective for several ion species Passive flux of sodium and potassium is balanced by active pumping of the ions Chloride ions may be passively distributed;静息膜电位;Ionic Basis of generation of membrane potential Nernst Equation Goldman-Hodgkin-Katz (GHK) equation Electrical model of cell membrane Electrical excitability and mechanism of generation of action potential Voltage clamp Patch clamp technique;§1.3 膜的电学特性;;;;Membrane potential Vm is the sum of equilibrium potentials (EX), each weighted by the ion’s fractional conductance.;The property of cell membrane as capacitator;Capacitance=Q/V;V0;Stimulus;Due to the existence of membrane capacitance, total membrane current has two components: ;Ionic Basis of generation of membrane potential Nernst Equation Goldman-Hodgkin-Katz (GHK) equation Electrical model of cell membrane Electrical excitability and mechanism of