国外大学细胞生物学英文5.bioy 培训资料.pptVIP

How is immunofluorescence done in a lab? W2011 D103 - lecture 5 @ Suetterlin and MacGregor - all rights reserved. * Fluorescent light excitation emission Light Nucleus microtubules Golgi Primary antibody (Rabbit anti tubulin Ab) recognizes antigen (eg tubulin) Secondary antibody (Goat anti-rabbit Ab) recognizes the constant region of rabbit antibodies Recognizes Golgi protein Fluorescent light Light more than one secondary antibody binds to one primary antibody ? signal enhancement antibody coupling is inefficient - precious primary antibody would be lost during the coupling process. fl