通过粪球形态快速鉴定马鹿性别的方法与分子鉴定 - 遗传.PDF

兽类学报,2010,30 (3):317 - 321 Acta Theriologica Sinica 通过粪球形态快速鉴定马鹿 性别的方法与分子鉴定 1  1,2 1,3 张明海 田新民 李熠 (1 东北林业大学野生动物资源学院,哈尔滨 150040)(2 牡丹江师范学院生物系,牡丹江 157012 ) (3 黑龙江省环境保护科学研究院,哈尔滨 150056) 摘要:通过 SRY 基因扩增,对76 份野生东北马鹿粪便 DNA 进行了性别鉴定,其中雄性49 份,雌性27 份。观 察发现粪球形态可分两类:子弹状、枣核状。子弹状呈短粗型,长宽比较小;枣核状呈细长型, 长宽比较大。 对 76 份样品进行分类:子弹状 51 份、枣核状 25 份。并以样品长、宽平均值的比值 (R ) 为指标快速聚类,并 建立了判别方程。统计指出判别结果与实际性别吻合率79 71% ;形态分类与实际性别吻合率 90 56% , 即子弹 状为雄性,枣核状为雌性。结果提示今后的野外研究可直接利用粪球形态判定粪样性别,长宽比判别方程可作 为辅助。 关键词:东北马鹿;粪球形态;SRY 基因;性别鉴定 中图分类号:Q958 文献标识码:A 文章编号:1000 - 1050 (2010 )03 - 0317 - 05 A molecular identification and rapid recognition approach for the sex de- termination of wild wapiti by feces pellet morphology 1  1 ,2 1 ,3 ZHANG Minghai ,TIAN Xinmin ,LI Yi (1 College of Wildlife Resources,Northeast Forestry University,Harbin 150040,China) (2 Department of Biology,Mudanjiang Normal College ,Mudanjiang 157012,China) (3 Heilongjiang Provincial Research Institute of Environmental Sciences,Harbin 150056,China ) Abstract :The DNA sex of wild wapiti was determined by PCR amplification of the SRY genes of 76 feces samples. Among of them ,49 samples were identified as male,and the others as female. All fecal pellets presented one of two shapes :bul- let-like and jujube-stone like. The bullet-like fecal pellets displayed a short thick form with a low length/ width ratio. The jujube-stone like ones displayed a thin long form with high length/ width ratio. Using these shapes,the 76 samples were di- vided into two groups :51 bullet-like and 25 jujube-stone li