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高效液相色谱法检测苦瓜啤酒中的苦瓜皂苷
438 2007, Vol. 28, No. 07 食品科学 ※分析检测
李 健,刘 鑫,刘 宁,陈姝娟,黄 艳
(哈尔滨商业大学食品工程学院, 黑龙江 哈尔滨 150076)
:建立了高效液相色谱法对苦瓜啤酒中功效成分——苦瓜皂苷检测的方法。以70% 乙醇超声辅助提取苦瓜
皂苷,大孔树脂纯化,得苦瓜皂苷纯品。以自制的苦瓜皂苷为标准品与苦瓜啤酒中的苦瓜皂苷进行比较,用 ODS-
3 柱,在205nm 下,乙腈和水(55:45,V/V)为流动相,流速1.0ml/min,经反相液相色谱分析,以保留时间定
性、峰面积定量。结果得出苦瓜啤酒中苦瓜皂苷的总含量为22.16μg/ml,相对标准偏差<4%,回收率在97.53%~
100.32% 之间。此方法重现性好且操作简单,是比较理想的检测方法。
:高效液相色谱法;检测;苦瓜啤酒;苦瓜皂苷
Detection of Bitter Melon Saponin in Bitter Melon Beer by HPLC
LI Jian,LIU Xin,LIU Ning,CHEN Shu-juan,HUANG Yan
(Institute of Food Sciences and Engineering, Harbin University of Commerce, Harbin 150076, China)
Abstract :Detection of efficient component in bitter melon beer was established by HPLC. The bitter melon saponin was
extracted by transonogram-assisted using 70% ethyl alcohol, then was purified by macroporous resin, and the straight of bitter
melon saponin was obtained. Bitter melon saponin in bitter melon beer was compared with standard substance made by our
laboratory. An RP-HPLC method was set up, using ODS-3 column, acetonitrile: water (55:45, V/V) as mobile phase with a flow
rate of 1.0 ml/min at the detection wavelength of 205 nm, the qualitative and the quantitative analyses were carried out based on
the retention time and peak area respectively. The results showed that the total content of bitter melon saponin in bitter melon
beer is 22.16μg/ml, RSD (relative standard deviation) is less than 4%, recovery is between 97.53%~100.32%. This method
is excellent reproducibility and the is operation simple. It is a perfect detection method.
bitter melon beer;bitter melon saponin
Key words:HPLC;detection;
中图分类号:TS255.1 文献标识码:A 文章编号:1002-6630(2007)07-04
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