樟树组织培养快繁育苗技术研究.pdfVIP

广东林业科 技 2007年第23卷第5期 35 樟树组织培养快繁育苗技术研究 龚 峥 周丽华 张卫华 黎新宇 (1．广东省林业科学研究院 广州 510520；2．广东省东江林场) 摘要 文章报道了应用组织培养育苗技术对樟树家系进行快速繁殖的初步结果。试验表明：带腋芽的 茎段外植体在添加细胞分裂素和生长素的MS培养基上可分化形成不定芽，但芽增殖和继代培养过程并不 需要添加生长素。在DCR+BA 3．0 mg／L的培养基上增殖培养时，平均每个外植体增殖4．7个不定芽，高度 小于1．5 cm的不定芽的比例为74．1％；而在DCR+BA 4．0 mg／L的培养基上培养时，平均每个外植体增殖 2．4个不定芽，高度大于1．5 cm的枝芽比例可提高至58．1％。枝芽接种在DCR+IBA 1．5 mg／L的培养基 上诱导生根培养28 d的生根率为98％，移植的再生植株95％以上存活。 关键词 樟树 组织培养 快速繁殖 中图分类号：$722．3 7 文献标识码：A 文章编号：1006—4427(2007)05—0035—05 Rapid Propagation of Cinnamomum camphora Via Tissue Culture Gong Zheng Zhou Lihua Zhang Weihua Li Xinyu (1．Guangdong Forestry Research Institute，Guangzhou，510520；2．Guangdong Dongjiang Forestry Farm) Abstract This paper deals with the preliminary results on tissue culture and rapid propagation of Cinnamo— mum camphora．The adventitious buds could be induced from shoot tips and axillary buds derived from family mate— rial MZMJ402 on MS medium supplemented with BA and NAA．Nevertheless，the trial results from subcultures in— dicated that the auxins had a disadvantageous effect on multiplication of adventitious buds．The optimum propaga— tion medium selected from the experiments were DCR containing BA 3．0 mg／L or 4．0 mg／L，in which the multiple coefficients reached 4．7 and 2．4 respectively．The trial identified that the optimum medium for root induction was DCR supplemented with IBA 1．5 mg／L and 98％ of rooting percentage was obtained．The plantlet survival rate could exceed 95％ provided that the nursery condition was acceptable． Key words Cinnamomum camphora(L．)Presl，tissue culture，rapid propagation 樟树(Cinnamomum camphora(L．)Pres1)又名香樟，樟科樟属，原产我国东南及西南各地。樟树为常绿乔 木，高大挺拔，是我国南方著名的珍贵乡土用材树种，国家二级重点保护植物 11。由于乡土树种的适生范围 广，在发展桉树和相思等短轮伐期工业用材林有局限性的地区，完全可以利用乡土树种来营造商品林和用材 林，因此樟树也就成为我省重点发展的乡土阔叶商品林树种。目前，我