第一章 3 B型流感耐药检测位点.pdfVIP

Antiviral Research 85 (2010) 354–360 Contents lists available at ScienceDirect Antiviral Research j o u rn al h omepage: w w w. elsev /locate/antiviral Detection of antiviral resistance and genetic lineage markers in influenza B virus neuraminidase using pyrosequencing Tiffany G. Sheu 1, Varough M. Deyde 1, Rebecca J. Garten, Alexander I. Klimov, Larisa V. Gubareva ∗ Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA a r t i c l e i n f o a b s t r a c t Article history: We report here the design of a pyrosequencing approach for the detection of molecular markers of resis- Received 30 July 2009 tance to the neuraminidase inhibitors zanamivir and oseltamivir in influenza viruses of type B. Primers Received in revised form were designed to analyze the sequences at eight amino acid positions E119, R152, D198, I222, S250, 29 September 2009 H274, R371, and G402 (universal A/N2 numbering) in the neuraminidase (NA) which have been previ- Accepted 22 October 2009 ously found to be associated with resistance or reduced susceptibility to oseltamivir and/or zanamivir in the NA inhibition assay. In addition, the designed primers could be utilized to the distinguish between the Keywords: NAs of influenza B viruses from the two major lineages (Victoria and Yamagata) that