利用spr 技术测定湖水中微囊藻毒素 - 北京玛格泰克科技发展有限公司.pdfVIP

中国环境科学 2012,32(7) ：1284~1287 China Environmental Science 利用 SPR 技术测定湖水中微囊藻毒素 1 1 1 1 2 1* 周宏敏 ,欧惠超 ,任 鹏 ,张海燕 ,王晓萍 ,罗昭锋 (1. 中国科学技术大学生命科学学院,合肥微尺度物质 科学国家实验室,安徽 合肥 230027 ；2.浙江大学光电信息工程学系,浙江 杭州 310027) 摘要：利用表面等离子共振(SPR)技术,建立了快速定量检测湖水中微囊藻毒素 LR(MC-LR) 的方法.将 MC-LR 共价偶联到表面等离子共振 芯片表面,检测了芯片的稳定性,并利用免疫竞争抑制原理构建标准曲线,对湖水样品进行了检测.结果表明:制备的芯片信号稳定,50 个循环 相对标准偏差(RSD)为 0.62%;该方法的最低检测浓度为 0.25ng/mL,可作为水体中 MC-LR 的分析检测方法. 关键词：表面等离子共振；芯片；微囊藻毒素；免疫竞争 中图分类号：X703.5 文献标识码：A 文章编号：1000-6293(2012)07-1284-04 1 A novel detection method of microcystin of lake water by surface plasmon resonance technique. ZHOU Hong-min , 1 1 1 2 1* OU Hui-chao , REN Peng , ZHANG Hai-yan , WANG Xiao-ping , LUO Zhao-feng (1.Hefei National Laboratory for Physical Sciences at Microscale, School of Life Sciences, University of Science and Technology of China, Hefei 230027, China ；2.Department of Optical Engineering, Zhejiang University, Hangzhou 310027, China). China Enviromental Science, 2012,32(7)：1284~1287 Abstract ：Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR (surface plasmon resonance) technique. After the MC-LR being immobilized on the surface of the chip, the stability of the chip was tested, and the standard curve was constructed by the principle of competitive immune inhibition. The results showed that the relative standard deviation of 50 cycles was 0.62%, which represented the stability of the immobilized chip. And the limited concentration of detection is arrived at 0.25ng/ml.. For its stability and sensitivity, the SPR detection method would be applied to as