阿加曲班对实验性大脑内出血后脑组织MMP-9表达变化的研究.pdfVIP

· 650· J Apoplexy and Nervous Diseases，December 2007，Vol 24，No．6 文章编号：i003—2754(2007)06—0650—03 阿加曲班对实验性大脑内出血后脑组织 MMP一9表达变化的研究 丛玉玮 ， 戚基萍 ， 段淑荣 ， 吴 鹤 ， 王丹丹 ， 孙玉兰 摘 要： 目的 研究大鼠实验性大脑内出血(ICH)后脑组织基质金属蛋白酶一9(matrix metall0pr0teinases一 9，MMP一9)的表达在阿加曲班(argatroban)注入前后的变化及意义。方法 采用立体定向技术将自体不凝血注入 大鼠尾状核区制备不同时间段的大脑内出血模型。另设加药组，术后30min及术后每天给予同等量的阿加曲班(3．0 mg／kg)。免疫组化染色法检测注药前后出血脑组织MMP一9的表达。结果 未加药组MMP一9表达的阳性细胞数在 出血后2d达到最高峰，之后又下降。加药后MMP一9表达的变化趋势消失(，】O．05)，同一时相MMP一9阳性表达的 细胞数与加药前无显著性差别(，】O．05)。结论 进行阿加曲班治疗后MMP一9表达无显著性改变，MMP一9在出血 后脑组织可能具有双重作用。 关键词： MMP一9； 凝血酶； 阿加曲班； 大脑内出血 中图分类号：R743．34 文献标识码：A Influence of argatroban no MMP—。9 expression after experimental intracerebral hemorrhage in rats CONG Yu—。 wei，QI。li—ping，DUAN Shu—rong，el a1．(Department of Pathology，the First Hospital of Harbin Medical Universi— ty，Harbin 15001，China) Abstract： Objective To investigate the influence of argatroban(a kind of direct thrombin inhibitor)no Ma— trix Metalloproteinases一9(MMP一9)expression in brain tissue at different time after experimental cerebral hemor— rhage in rats．Methods Stereotactic apparatus and autologous blood was infused into the left caudate nucleus tO build up rat experimental Intracerebral hemorrhage(ICH)mode1．Treatment with argatroban group was the given epactally argatroban 3．Omg／kg 30min and once a day after opteration．Results The expression of MMP一9 peaked at 2d，then decreased．While treated with argatroban，the express tendency of MMP一9 was disappeared，and the ex— pression of MMP一9 did not significantly down—regulate by argatroban．Conclusion The expression of MMP一9 was not significantly changed by argatroban．MMP一9 may had very different roles in tissue after ICH． Key words： Matrix metallopmtejnases一9(MMP一9)； Thrombin； Argatroban； Intraeerebral hemo