枯草芽孢杆菌冷冻干燥保护剂的初步研究.docVIP

枯草芽孢杆菌冷冻干燥保护剂的初步研究 摘 要：为提高枯草芽孢杆菌菌粉冻干存活率，对其冻干保护剂进行筛选和优化。采用单因素筛选试验和正交试验，通过分析菌粉冻干存活率，筛选优化出最佳复合保护剂的组成。最终确定枯草芽孢杆菌最佳冻干保护剂的配方为：脱脂乳粉10%、L-抗坏血酸钠3%、海藻糖2%，菌种冻干存活率高达(90.01±1.31)%。本研究对枯草芽孢杆菌菌种的保藏、应用、活菌产品质量的稳定以及新产品的开发均有一定的理论指导意义。 关键词：枯草芽孢杆菌；冻干保护剂；正交设计；冻干菌粉 Preliminary Research on the cryoprotectant of Bacillus subtilis Powder Abstract：technology of selection and optimization of Bacillus subtilis freeze-dried powder, in order to improve the survival rates of Bacillus subtilis. The single factor and orthogonal test were taken, through analysis of?the survival bacteria, and then optimized?the best form of comp