固相萃取-高效液相色谱法优化测定饮用水中的微囊藻毒素.pdfVIP

146 广东农业科学 2014 年第 l 期 回相萃取-高效液相色谱法优化测定 饮用水中的微囊藻毒案 郭伟，黑壳，杨燕婷，雷列辉，朱小平 (珠江水利委员会珠江水利科学研究院，广东广州 510611 ) 摘 要:对流动相酸度、洗脱速度和色谱柱柱温进行了优化，建立了→个非常适用于检测饮用水中微囊藻毒素 LR 的分析方法。该方法用 O.04%TFAI乙睛(63:37) 为流动相，洗脱时间为 7 min。该方法具有温和、快速、经济、适用等 特点。经过对广东省某典型水库的微囊藻毒素进行检测分析，结果表明:该方法定量可靠、准确、灵敏，回收率为 889岛，最低检出限为 0.05μg/L o 关键词:固相萃取;高效液相色谱;优化;测定;微囊藻毒素 中图分类号:X13 1.2 文献标识码:A 文章编号:1 ∞4-874X(2014)01-0146-03 Optimization and detection of microcytin in drinking water using solid phase extraction-high performance Iiquid chromatography GUO Wei , HEI Liang , YANG Yan一ting， LEI Lie-hui , ZHU Xiao-ping (Pearl River Hydraulic Research lnstitute, Pearl River Wαter Resource Commission, Guangzhou 510611 , China) Abstract: This study established an available analysis method for the detection of microcystin LR in drinking water. ηle mobile phase acidity, elution rate and column temperature were optimized in this method. 0.04% TFA/acetonitrile (63: 37) was chosen as the mobile phase , elution time was 7 min. The method was gentle , fast , economical , available etc. Through the detection and analysis of microcystin LR in a typical reservoir of Guangdong province , we found that this method was reliable , accurate , sensitive, and the recoveηwas 889毛， the lowest detection limit was 0.05μglL. Key words: solid phase extraction; high perfo口nance liquid chromatography; optimization; detection; microcystin 种污染我国饮用水日益缺乏，罪魁祸首之一是微囊藻 湖泊、水库和河流中累积过多的氮、磷等高营养 毒素。我国在饮用水标准中明确规定，饮用水中微囊 物质，可使水体的生态结构与功能发生变化，导致藻