国槐植株再生技术研究.pdfVIP

安徽农业科学，Journal ofAnhui A ．Sci．2007，35(35)：11418—11419 责任编辑 姜 丽 责任校对 王淼 国槐植株再生技术研究 袁秀云，张仙云，马杰，桑慧敏 (郑州师范高等专科学校生物技术研究所，河南郑州45oo44) 摘要 『目的1筛选国槐组织培养的最佳培养基，建立国槐植株再生体系。『方法】将灭过茵的国槐种子分别接种到4种无菌苗诱导培 养基上，待苗长出2~4片真叶时，将叶、下胚轴、子叶分别接种于4种愈伤组织诱导培养基上，然后将子叶产生的愈伤组织分别接种 在2种芽分化培养基中，再将继代培养获得的有效苟转入生根培养基，对国槐种子进行无菌苟萌发和植株再生的研究。『结果1培养 基的激素配比对种子萌发的影响不大；6一BA能促进愈伤组织的产生；最适宜的胚状体诱导培养基为MS+2．0mg／L 6-BA+0．2mg／L NAA+o．5 mg／L KT；芽诱导培养基MS+6—1．0 mg／L BA+O，1 mg／I|NAA；生根培养基为1，2 MS+0．3 mg／L NAA，活性炭不利于国槐的生根 培养。r结论1种子的萌发率与国槐的种子质量有关，与培养基的种类关系不大。子叶最适合诱导愈伤组织。 关键词 国槐；组织培乔；愈伤组织 中图分类号 Q813．7+2 文献标识码 A 文章编号 0517—661l(2oo7)35—11418—02 Research on Tissue Culture and Regeneration Technology of Sophorajaponica YUAN Xiu-yun et al(Institute of BiologY Technology，Zhengzhou Teachers College，Zhengzhou，Henan 450044) Abstract 【Objective】The study aimed to screen out the optimum media for the tissue culture of Sophora japonica and set up its plant regeneration system．【Method】The sterilized S．japonica seeds were inoculated resp，on 4 kinds of induction media for inducing asepsis seedling， till the seedlings grew out 2~4 leaves，the leaves，hypocotyl and cotyledon were inoculated resp．on 4 kinds of induction media for inducing callus， then the callus grown out from cotyledon were inoculated resp，on 2 kinds of bud differentiation media，finally the effective seedlings obtained through subculture were transplanted into rooting media SO as to study the asepsis seedlings germination and plant regeneration of S，japonica seeds．【Result】The hormone matching of media had little influence on seed germination．6-BA could promote the production of callus，The most suitable induction media Was MS+2．0 mg／L 6-BA+O．2 mg／L NAA+O．5 mg／L KT for somatic embryo．and MS+6—1．0 mg／L BA+O．1 mg／L NAA for bud．The rooting media was 1／2 MS+O．3 mg／L NAA．Active carbon went against the rooting culture of japonica．【Conclusion]The seed germination rate relates to seed q