应用组织芯片技术研究人类胰腺癌中钙结合蛋白表达的生物学意义.pdfVIP

南方医科大学学报(J South Med Univ) 应用组织芯片技术研究人类胰腺癌中钙结合蛋白表达的生物学意义 杨文彬，王永恒，蔡 峰，曹 罡，秦兆寅(西安交通大学医学院第二附属医院普通外科。陕西 西安 710004) 摘要：目的探讨钙结合蛋白在胰腺癌中的表达及生物学意义。方法用组织微阵列技术构建包含5l例导管腺癌，3例胰 岛细胞癌，6例慢性胰腺炎患者，3例正常胰腺组织的63点阵的石蜡组织芯片。用免疫组化 SP法检测该芯片中钙结合 蛋白的表达，分析其与胰腺癌临床病理因素的关系。结果 5l例胰腺癌患者中，Calcyclin阳性表达率为76．5％(39／51)． 与正常组比较Calcyclin表达与胰腺癌显著相关()(2=9．714，P=-0．007)，而慢性胰腺炎时Calcyclin亦有表达，但慢性胰腺 炎组与正常组比较无明显相关性，同时胰腺癌组及慢性胰腺炎组间亦无明显相关性，Calcyclin表达与年龄、性别、组织 分化程度及TNM分期无明显相关性。结论Calcyclin与胰腺癌相关，可能与胰腺癌发生发展有密切关系。 关键词：胰腺癌；组织芯片；钙结合蛋白；免疫组化 中图分类号：Q78 文献标识码：A 文章编号：1673-4254(2007)12．1908．03 Biological significance of expression of calcyclin in human pancreatic carcinoma： a tissue microarray-based study YANG Wen-bin，WANG Yong-heng，CAI Feng，CAO Gang，QIN Zhao—yin Department ofGeneral Surgery,Second Affiliated H0sp l，School ofMedicine，Xi’all Jiaotong University，Xi’all 710004，China Abstract：Objective To investigate the expression ofcalcyclin in pancreatic carcinoma and its relation to the patients’prognosis． Methods Human pancreatic carcinoma tissue microarray was constructed,which contained 63 cores of3 normal adult pancreas tissues,6 chrome pancreatitis tissues，5 1 pancreatic c~cinoma tissues and 3 islet cell carcinoma tissues．Immunohistochemistry Was performed to det0ct the expression of calcyclin in these tissues． and the relationship between calcyclin and the clinicopatholoical features of pancreatic carcinoma was analyzed． Results The positivity rate of calcyclin in the pancreatic carcinomatissuewas 76．5％(39／51)，and calcyclin stainingwasmoreintenseinthemalignant cellsthanintheb~ign cells( = 0．007)，suggesting a correlation between calcyclin expression and pancreatic carcinoma No evidence was found for an association ofcalcyclin expressionwitl1thevariablesinc~dingthepatients’gender,age at surgery,andtumorgrade．Weak stainingfor calcyclin was noted in chronic pancreatitis tissues． Conclu