水稻RPR1启动子片段对烯丙异噻唑诱导响应性的分析.pdfVIP

524 JournalofPlantPhysiologyandMolecularBiology 2007，33(6)：524-530 Analysis of Probenazole·Resp0nsiVeness of Rice RPR1 Upstream Fragments ZHAO Xiang-Hui，YANG Jin—Xiao，GAO Jiong，ZHOU Qian，KUAI Ben．Ke Institute ofPlant Biology，School ofLife Sciences，Fudan University，Shanghai 200433，China Abstract：RPR1(rice probenazole—responsive)is a rice grisea，induces SAR—like disease resistance in rice and gene，the expression of which is responsive to SAR in tobacco and Arabidopsis(Sakam oto et a1．1 999； probenazole(PBZ)，a synthetic compound that may act Nakashita et a1．2o02：Y0shioka et a1．2o01)． as aplantdefenseactivator．Ithasbeen shownthatRPR1 RPR1(rice probenazole—responsive)is a gene may be involved in disease resistance responses．In probenazole—responsive gene in rice．RPR1 contains a this study，a series ofamplified fragments from therice nucleotide—binding site and leucine．rich repeats．thus RPRl promoter region，including 2 416 bp，1 574 bp， sharing structural similarity with known disease resis． 819 bp，568 bp and 208 bp fragments upstream to the tance genes．111e expression of尺尸 ．，in rice can be up— ATG translation start site，were prepared and linked to regulated by treatment with chemical inducers of SAR the coding region of~-glucuronidase(GUS)gene．Analy— or by inoculation with pathogens．The mode of RPR1 sis of GUS gene transient expression in rice calli dem— expression in probenazole—treated rice correlates well onstrated that the 568 bp fragment was sufficient for with the induction of blast resistance，indicating the par- probenazole responsiveness．Analysis of GUS gene stable ticipation ot RPRl in induced disease resistance expression in Arabidopsis thaliana indicated that the (Sakamoto et a1．1 999)．