白菜雄性不育系及保持系的花器官和抗氧化酶活性的差异.pdfVIP

浙江农业学报Acta Agricuhurae Zhejiangensis 19(6)：404～408，21307 白菜雄性不育系及保持系的花器官和抗氧化酶活性的差异 王玲平，胡天华，包崇来，胡海娇，戴丹丽，毛伟海 (浙江省农业科学院蔬菜研究所，浙江杭州310021) 摘 要：以3个遗传背景不同的父本转育而成的白菜不育系(04．256，04．320，04．376)及其相应的保持系(04- 34，04-56，04．102)为材料，观察花蕾和花药等花器官的形态变化；同时对其开花期的花蕾、花茎、花瓣、雌蕊和 叶子的ASA．POD(抗坏血酸过氧化物酶)，POD(过氧化物酶)，CAT(过氧化氢酶)，SOD(超氧化物歧化酶)等抗 氧化酶活性进行测定。结果表明：(1)3份不育材料不育度均为100％，不育系花蕾纤细、瘪，雄蕊乳白色、退 化成短丝状、无花粉；(2)各器官的ASA．POD，POD，CAT，SOD等抗氧化酶的活性变化在开花期表现基本一致， 花蕾的抗氧化酶活性不育系高于保持系，而雌蕊的酶活性表现则相反，抗氧化酶活性保持系均高于不育系， 且每个对应的不育系和保持系材料问均达显著差异；保持系和不育系的花茎、花瓣、叶子的抗氧化酶活性不 同。 关键词：白菜；不育系；保持系；花器官；酶活性 中图分类号：$634．3 文献标识码：A 文章编号：1004—1524(200r7)o6—04O4—05 Differences of both floral organs and antioxidant enzyme activities in maintainer lines and sterile lines of Brasswa campestris L．ssp．chinensis H砭Il var．communis WANG Ling-ping，HU Tian—hua，BAO Chong-lai，HU Hai—jiao，DAI Dan—li，MAO Wei—hai (Institute ofVegetables，ZhejiangAcadeamy ofAgricultural ，Haugzhou 310021，China) Abstract：We carried out ejther observations of both buds and anthers or determinations of ASA—PO D，PO D，CAT，SO D in buds，scapes，petals，pistil and leaves of three sterile lines(04-256，04-320，04-．376)and their corresponding maintainer lines(04_3_4，04-56，04-102)during flowering period．The results show that(1)the three sterile lines，slight and shrunken in buds，ivory-white and short—silky in pistils were steadily 100％of male-sterile ratio；(2)the trends of ASA-POD，POD， CAT，SO D activities in the floral organs tested were consistent during anthesis．Antioxidant enzyl／le activities in buds were higher in sterile lines than maintainer lines，whereas antioxidant enzyme activities in pistils vice versa．There ale significant difference in activity of all 4 enzymes between sterile lines and their corresponding maintainer lines．Antioxidant enzyme ac— tivities in stapes，petals，leaves exhibit different trend in s