糖尿病早期大鼠心肌组织中胶原纤维的改变与醛糖还原酶的相关性及木犀草素的保护作用.pdfVIP

齐齐哈尔医学院学报200i年第28卷第2l期 · 2569 · 糖尿病早期大鼠心肌组织中胶原纤维的改变与 醛糖还原酶的相关性及木犀草素的保护作用 陈小萍 徐 丽 陈海英 【摘要】 目的 探讨糖尿痛(diabers mellitus．DM)早期大鼠心肌组织中胶原纤维(collagen fibers， CF)与醛糖还原酶(Aldose Reductase，AR)的相关性及木犀草素(I|uteolin)的保护作用。方法 应用链 脲佐菌素(streptozotocin，SI’z)左腹腔注射诱发糖尿痛，以木犀草素灌胃治疗。分为正常对照组、糖尿 病模型组和木犀草素治疗组。分别于3od和 60d测定各组大鼠匀浆中醛糖还原酶含量，并利用Van Gieson氏胶原纤维染色法观察各组大鼠心肌胶原纤维。结果 DM大鼠心肌匀浆中的醛糖还原酶较正 常组显著升高，经木犀草素治疗后心肌匀浆中醛糖还原酶(AR)降低，糖尿病30d和60d心肌组织中胶 原纤维增多变粗，木犀草素治疗后胶原纤维变细、减少。结论 糖尿痛早期大鼠心肌组织中胶原纤维的 改变与醛糖还原酶量的变化有关，木犀草素能通过抑制醛糖还原酶(AR)活性而保护心肌细胞。 【关键词】 醛糖还原酶 木犀草素 糖尿痛 链脲菌素 Correlation between collagen fibers changes of cardiac muscle and aldose reductase in cardiac muscle of early diabers rats and protection or luteolin on them Chert Xiaoping，et al、 (Department of Biochemistry， Putian University，Putian，Fuiian 351100 China) [Abstract] Objective To investigate the correlation between collagen fibers changes of cardiac muscle and aldose reductase in cardiac muscle of early diabers rats and protective effects of 1uteolin on them．and aldose reductase in cardiac muscle of early diabers rats and protective effects of 1uteolin on them．Methods SD rats were divided into normal group、diabers group and group treated with luteolin． Modal rats were injected into intraper oneal1y by streptozotocin(STZ)．The content of AR in cardiac muscle of all rats were quantified after 30d and 60d，the collagen fibers Were observed by Van Gieson col— lagen fibers dye．Results The content of AR in cardiac muscle increase evidently in DM groups．collagen fibers in cardiac muscle were more in DM groups than the normal group．The quantiry of ARin cardiac muscle of diabers rats treated with 1uteolin were 1ower than diabers rats．The collagen fibers decrease ev— idently in group treated with luteolin．Conclusions The collagen fibers changes in cardiac muscle of early diabers rats are related to the increasing content of AR，which is able to p