10621_2009044019吴超 - 成都信息工程大学论文管理系统.docVIP

分类号：X832 U D C：D10621-408-(2013)2782-0 密 级：公 开 编 号：2009044019 成都信息工程学院 学位论文 论文作者姓名： 申请学位专业： 环境 申请学位类别： 学学士 指导教师姓名（职称）： （教授） 论文提交日期： 201年0月日 钙黄绿素荧光光谱法测定环境水中氟 摘要 通过实验发现，钙黄绿素在KH2PO4—NaOH缓冲溶液中能与锆离子（Zr4+）形成络合物，并且具有一定的荧光强度。当向该络合物溶液中加入一定量的氟离子后，由于氟离子能夺取络合物中的锆离子（Zr4+）形成更稳定的配合物，使其荧光强度减弱，从而产生荧光猝灭现象。据此，建立了基于荧光猝灭测定氟离子含量的新方法。对钙黄绿素浓度、锆离子浓度、反应时间、温度等可能影响体系荧光强度的因素进行了考察，得到了优化的最佳实验条件。结果表明，在激发波长为λex=504nm和发射波长为λem=520nm条件下，氟离子在0.3——2.0mg/L的浓度范围内与荧光猝灭信号呈现良好的线性关系，相关系数为0.9931，检出限为0.094mg/L，对浓度为1mg/L的氟离子平行测定五次的相对标准偏差为5.1%。将该方法应用于环境水样中氟离子的检测，加标回收率在86.2%--93.3%之间，表明该方法可以用于测定含氟废水的测定。 关键词 钙黄绿素；锆离子；荧光猝灭法；氟 Determination of fluoride in water samples by Calcein fluorescence spectrometry Abtract Through experiments, a complex could be formed by the reaction of calcein and zirconium ions (Zr4+) in KH2PO4 - NaOH buffer solution, which has stronger fluorescence signal. When the fluoride was injected into the complex solution, the fluorescence signal was reduced because of the substitution of calcein by fluoride in the complex. Based on fluorescence quenching is established on this basis, a new method for determination of fluoride ion content. Concentration of calcein, zirconium ions concentration, reaction time, temperature, etc on the fluorescence intensity factors may affect the system, optimize the optimum experiment conditions are obtained. Results show that the excitation for λex = 504 nm wavelength and emission wavelength for λem = 520 nm condition, fluorine ion in 0.3-2.0 mg/L range of concentration and fluorescence quenching signal show good linear relation, the correlation coefficient is 0.9931, the detection limit of 0.094 mg/L, the concentration of 1 mg/L of fluorine ion determination of five times the relative standard deviation of 5.1%. The method was applied to analysis of fluorine ions in environmental water samples, the standard addition recovery rate was between 86.2% - 93.3%, show that the method can be used for the de