氯化血红素对cds 量子点荧光的猝灭研究及分析应用 - 食品科学.pdfVIP

※基础研究 食品科学 2011, Vol. 32, No. 03 51 CdS 张文龙，张俊生，周丽萍，陈莉华 * (吉首大学化学化工学院，湖南 吉首 416000) 以巯基乙酸为稳定剂及表面修饰剂，在水溶液中合成了平均粒径为2.9nm 左右的 CdS 量子点，该量子点 在 512.4nm 波长处有强的源于表面阱的空穴电子重组形成的表面态发射峰，加入的氯化血红素通过扩散和碰撞作用 可将电子转移至 CdS 量子点的空穴中导致量子点荧光发生动态猝灭，并在此基础上建立了测定氯化血红素的新型荧 光分析法。在最佳测定条件下，当氯化血红素的质量浓度为 5.0 ×10-6 -6 ～25.0 ×10 g/mL 时，体系的相对荧光强度 (F)与氯化血红素的质量浓度( -6 -8 ρ)呈线性关系，线性回归方程为：F=719.09 －14.01 ×10 ρ，检出限为3.35 ×10 g/mL 。 测定血清样品基底中氯化血红素的含量并获得满意结果。 CdS 量子点；氯化血红素；电子转移；荧光猝灭 Fluorescence Quenching Mechanism of CdS Quantum Dots by Hemin and Its Application ZHANG Wen-long ，ZHANGF Jun-sheng ，ZHOU Li-ping ，CHEN Li-hua* (College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China) Abstract ：CdS quantum dots were synthesized in aqueous solution by using mercaptoethylic acid as the stabilizer and surface -modification reagent. The 512.4 nm fluorescence emission which was caused by the cavity-electron recombination on surface trap of CdS quantum dots was quenched in the presence of hemin. The quenching mechanism was a dynamic process based on 2+ -6 electron transfer between Fe of hemin and cavity of CdS quantum dots. Under optimal conditions, a concentration of 5.0 ×10 － -6 g/mL of hemin could be determined on the basis of the decrease ratio of fluorescence intensity of CdS quantum dots, 25.0 ×10 -8 g/mL. The proposed method was utilized to analy