大豆细胞质雄性不育恢复基因的SSR标记.pdfVIP

第z6卷 第6期 大 豆 科 学 VoL 26 No。6 2007年 1Z月 S0YBEAN SCIENCE Dec． 2007 大豆细胞质雄性不育恢复基因的SSR标记 赵丽梅，王玉民，孙 寰，赵洪锟，程延喜，彭 宝，王曙明，张伟龙，董英山 (吉林省农业科学院大豆研究中心，长春 130033) 摘要 大豆细胞质雄性不育系的获得，为大豆杂种优势利用奠定了基础。在确认 RN型大豆细胞 质雄性不育系属单基因配子体不育，恢复性是由显性单基因控制的遗传模式的基础上，开展恢复基 因的分子标记研究，旨在找到与恢复基因紧密连锁的分子标记，为进一步克隆恢复基因及恢复系的 分子标记辅助育种奠定基础。研究选用412对SSR引物，利用RN型不育系YA与恢复系167杂 交的F 分离群体，获得了与恢复基因连锁的两个标记Satt414和Satt596，遗传距离分别为16．4和 14．6 cM。为了找到更近的分子标记，分析了Satt414和 Satt596附近的所有 SSR引物，并利用两 个遗传差异较大的亲本重新构建了分离群体，从而获得了与恢复基因连锁比较紧密的标记 Satt547，遗传距离为7．56 cM。根据Cregan等构建的大豆分子遗传连锁图，将恢复基因定位于J 连锁群上。 关键词 大豆；细胞质雄性不育；恢复基因；分子标记 中图分类号 S565．1 文献标识码 A 文章编号 1000—9841(2007)06—0835～05 IDENTIFICATIoN oF SSR MARKERS LINKED To THE FERTILITY RESToRER GENE FoR THE CMS IN SoYBEAN ZHAO Li—mei，WANG Yu—min，SUN Huan，ZHAO Hong—kun，CHENG Yan—xi，PENG Bao，WANG Shu- ming，ZHANG Wei—long，DONG Ying—shan (Soybean Research Center，Jilin Academy of Agricultural Sciences，Changchun 130033) Abstract The development of cytoplasmic male sterile(CMS)1ine has facilitated the utilization of soybean heterosis．It was confirmed that the fertility restoration of the RN type soybean CMS 1ine was controlled by a single dominant nuclear gene．Two SSR makers，Satt414 and Satt596，which were genetically linked to the nuclear restorer gene，were selected by screening of SSR markers with F2 segregation population from the cross between sterile 1ine YA and restorer 1ine 1 67．The genetic distance between the markers and the restorer gene were 1 6．4 and 1 4．6 cM，respectively． A segregating population developed from a cross between more diversified parents was used for screening the closer SSR markers．SSR marker Satt547 with genetic distance of 7．56 cM to the restorer gene was identified．The nuclear restorer gene was located in J linkage group ac