应用组织微阵列技术检测MMP-2和TIMP-2在卵巢肿瘤中的表达.pdfVIP

世界肿瘤孝专 2007年 第6卷 第4期 Tumour Journal oftheWorld Volume 6，Number 4，Dec 2007 ·259· 文章编号：1 683．0342(2007)04．0259．03 木论 著 木 应用组织微阵列技术检测MMP一2和TIMP一2在卵巢 肿瘤中的表达 王喜梅‘，舒筱灿‘，孙 雷 ，郑仁恕 摘要：目的 探讨基质金属蛋白酶．2(MMP．2)和组织金属蛋白酶抑制剂 2(TIMP．2)在卵巢肿瘤中 的表达。方法 应用组织微阵列技术结合免疫组化方法检测94例卵巢肿瘤组织中MMP．2和TIMP．2 的表达，以胞质出现棕黄色颗粒为阳性。结果 卵巢癌组织和卵巢交界性肿瘤组织中MMP．2阳性表达 率明显高于卵巢良性肿瘤(P0．05)，卵巢癌组织中TIMP．2阳性表达率明显高于卵巢良性肿瘤(P0．05)； MMP-2在IⅡ～Ⅳ期卵巢癌中的阳性表达率明显高于I～II期(P0．05)，MMP．2在病理分级2~3级阳性 表达率明显高于 l级(P0．05)；TIMP．2在不同临床分期和病理分级的卵巢癌中的阳性表达情况与 MMP．2相反。结论 MMP．2的表达上调和TIMP．2相对弱表达在卵巢恶性肿瘤的演进发展中具有重要 作用。 关键词：卵巢肿瘤；基质金属蛋白酶．2；组织金属蛋白酶抑制剂．2；免疫组化 中图分类号：R737．31 文献标识码：A Expression of MMP-2 and TIMP-2 in ovarian tumor by tissue microarray WANG Xi—mei ， SHU Xiao·can ，SUN Lei ，ZHEN Ren—SU ．rJ．Department of Basical Medicine，Huaihua Medical College， Huaihua 418000；2．Center ofPathology，Dalian Medical University，Dalian 116027,ChinaJ Abstract：Objective To study the expression of marx metalloproteinase··2(MMP··2)and tissue inhibitor of metalloprotemase-2(TIMP-2)in ovarian tumors Methods The expression of MMP-2 and TIMP-2 in 94 cases of ovarian tumor was determined by tissue microarray combined with inmunohistochemistry．The positive result was determined by the brown pellet in cytoplasm．Results The positive rates of MMP-2 in borderline tumors and ovarian cancer were significantly higher than that in benign neoplasms( 0．05)．The positive rate of TIMP-2 in ovarian cancer was also significantly higher than that in benign neoplasms(P0．05) The positive expression of MMP-2 in grade III～Ⅳ adenocarcinoma was significantly higher than in grade I～II(P0．05)，MMP-2 levels was significantly higher in G2—3 than in Gi．TIMP-2 expression was contrary to the expression of MMP-2 on tumor stage and differentiation．Conclusions Th e up-regulation of MMP-2 and weak expression of TIMP-2 may play an important role in the development of malignant ovarian tumor~ Key words：Ovarian