毒鼠强对鼠心肌、骨骼肌的作用及与血清酶升高的关系.pdfVIP

· 1268· 华急诊医学杂志2OO7年12月第16 Med，December 200r7，V01．16．No．12 · 基础研究 · 毒鼠强对鼠心肌、骨骼肌的作用及与 血清酶升高的关系 张慧利 余为治 黄亮 康秀文 【摘要】目的 研究毒鼠强对鼠心肌、骨骼肌的影响及与血清酶升高的关系。方法 用同种大 鼠30只，将其分成3组：对照组、半致死量组 (0．02mg／kg)及致死量组 (0．04mg／kg)；每组雌雄 各半。按大鼠的体重将毒鼠强粉溶于生理盐水后灌入胃内，对照组则灌入等量的生理盐水。于死亡 立即或1 h后取血清测心肌肌钙蛋白rIⅥ及肌酸激酶 (CK)、天门冬酸氨基转移酶 (AsI1)、乳酸脱 氢酶 (LDH)、a一羟丁酸脱氢酶 (a-HBDH)，并取死亡或处死大鼠的心肌和大腿骨骼肌做病理检查。 结果 大鼠中毒后10～60 min出现四肢或全身抽搐；致死量组6只大鼠于中毒后20—6o分钟死亡。 血清心肌肌钙蛋白 nd中毒组较对照组增高 (P0．01)，致死量组较半致死量组增高 (P0．01)； CK、AST、LDH、a-HBDH中毒组高于对照组 (P0．01)，致死量组高于半致死量组 (P0．01)； 中毒大鼠心肌、骨骼肌组织均有变性、坏死；中毒剂量越大，损伤越明显；骨骼肌的损害比心肌损 害更严重的。结论 毒鼠强急性中毒后，血清酶学异常升高；其心肌和骨骼肌组织均有显著损伤。 异常升高的血清酶是毒鼠强对心肌和骨骼肌双重损伤的结果。 【关键词】毒鼠强；心肌；骨骼肌；血清酶 Effects oftetrmni~ on cardiac／skeletal musde in the rats and elucidate the re蜘i0 ofthe effects and the elevated sero-enzyme ZHANG Hui-li，YU Wei-zhi，HUANG Hang，KANG Xiu-wen．Department of Emergency Medicine，The First affiliated Hospital ofNanchang University，Nanchang 330006，China 【Abstract】Objective To investigate the effects of tetrmnine to cardiac／skeletal muscle in山e rats and elucidate the relationship ofthe effects and the elevated sero-enzyme．Method The 30 homogeneous SD rats were invided into three groups：the control group．the half-lethal dose group and the lethal dose group．111e number of female and male rats was equa1． e tetramine powder is dissolved into 0．9％NS and poured into the stomach of the objects in medication groups；0．9％NS Was poured into the rats of control group．Once the rat died or one hours later，the related sero-enzyme Was determined and the rat was executed．Immediately cardiac muscle and skeletal muselewas respectively drawn the materials from the rat and Was pathologically examined．Results After the rats are intragastric administrated they spasm in 10-60 min；the 6 rats of the medial lethal dose group die in 20-60 min．Serum 1nL／CK／ASr／IlD