栀子苷、黄芩苷、华蟾酥毒基对体外培养的B16恶性黑素瘤细胞细胞增殖的抑制作用.pdfVIP

Chin J Dermato Venerol Integ Trad W Med 2007，Vo1．6 No．4 · 论 著 · 栀子苷、黄芩苷、华蟾酥毒基对体外培养的 B 1 6恶性黑素瘤细胞细胞增殖的抑制作用 李雅琳，焦振山，张玉环，刘栋 (天津市长征医院，天津 300120) 摘要：目的 观察栀子苷、黄芩苷、吴茱萸内酯、穿心莲内酯、华蟾酥毒基等5种中药单体成分抗恶性黑素瘤的 活性。方法 以系列浓度的上述5种药物作用体外培养的B16恶性黑素瘤细胞72 h，顺铂为对照，MTr法测定细胞 增殖抑制率。结果 在5种中药单体中，栀子苷、黄芩苷、华蟾酥毒基对体外培养的B16恶性黑素瘤细胞增殖有抑制 作用。其中栀子苷对细胞增殖的抑制率呈量效关系，最大抑制率达62．9％；黄芩苷仅在最高浓度时(80 I~g／mL)对细 胞增殖有抑制作用，抑制率为42．8％；华蟾酥毒基浓度≥20 lrg／mL时表现出对细胞增殖的抑制作用，但无明显量效 关系，最大抑制率仅为24．1％(4o lrg／mL)；80 lrg／mL的栀子苷对细胞增殖的抑制作用与2 lrg／mL的顺铂无明显差 异；穿心莲内酯、吴茱萸内酯则对细胞增殖无明显影响。结论 栀子苷、黄芩苷、华蟾酥毒基具有抗恶性黑素瘤的作 用，可以做为治疗恶性黑素瘤的备选药物。 关键词：中药；用效单体；恶性黑素瘤；B16恶性黑素瘤细胞；化学疗法 中图分类号：R739．5 文献标识码：A 文章编号：1672—0709(2007)04—0206—04 Inhibition of Proliferation in B16 Melanoma Cells by Jasminoidin，Baicalin and C Inobufagin LI Ya-lin,JIA0 Zhen-sholt,zHANG Yu—huan,LIUDong Ti,~jin Changzheng Hospital,Tianjin 30ol20．China Abstract：0bjective To investigate the antitumor activities of several natural product compounds in B16 melanoma ceUs in vitro．Methods Jasminoidin，baicalin，C inobufagin，andrographolide and limonin were incubated with cultured B16 melanoma cells at serial concentrations for 72 h．Inhibition of proliferation in cells were tested by MTr assay．Results Jas． minoidin．baicalin and C inobufagin induced cell growth inhibition whereas andrographolide and limonin had no significant effects on cell prohferation．Jasminoidin inhibited proliferation of cells in a dose—dependent manner and the maximum inhibit rate Was 62．9％at 80 p~g／mL．Baicalin inhibited cell growth only at the highest concentration f80 I~g／mL)with the 42．8％sup- pression of proliferation．C inobufagin induced cell growth at the concentration 20 p~g／mL or higher with the maximum inhibit rate 24．1％． No significant different in suppression of proliferation Was observed between 80~g／mL jasminoidin and 2~g／mL cisplatin(contro1)．Conclusion Jasminoidin，baicalin and C inobufagin induced cell growth