临床型乳房炎奶牛乳清差异蛋白质的双向电泳图谱分析.pdfVIP

2007年12月 甘 肃 农 业 大 学 学 报 第 4 2卷 第6期41～44 JOURNAL OF GANSU AGRICULTURAL UNIVERSITY 双 月 刊 临床型乳房炎奶牛乳清差异蛋白质的双 向电泳图谱分析 何 渊，杨永新，赵兴绪 (甘肃农业大学动物医学院，甘肃 兰州 730070) 摘要：采用双向凝胶电泳技术和计算机辅助图象分析方法，对临床型乳房炎奶牛和健康奶牛的乳清蛋白质进 行分析和比较，结果表明：在等电点为4～8。相对分子量为15 000—75 000 kD的区域内，临床型乳房炎奶牛乳清中 有(128=t=8)个蛋白斑点。而健康奶牛乳清中有(122±4)个蛋白斑点．对临床型乳房炎奶牛与健康奶牛乳清蛋白质 图谱匹配比较发现，有31个蛋白质点表达存在明显差异。其中乳房炎乳清中有 7个蛋白点表达发生明显上调。6个 蛋白点表达发生明显下调．4个蛋白点没有表达。并且乳房炎乳清新出现了1O个蛋白点． 关键词：乳房炎．孚L清；双向电泳；蛋白质组图谱 中图分类号：S 823．9 i 文献标识码：A 文章编号：1003—4315(2007)06—0041—04 Identification of differential wheyprotein trom healthy and clinic ‘ frc healthy and ：linic mastitis COWS by two—dimensional gel e1ectrophores s HE Yuan，YANG Yong-xin，ZHAO Xing—XU (College of Veterinary Medicine，Gansu Agricultural University，Lanzhou 730070。China) Abstract：Two-dimensional gel electrophoresis technique and computer assisted image analysis were used to separate the protein spots and analyze the different expression of bovine whey from healthy and those with clinical mastitis．The results showed that(128±8)protein spots were detected in whey from those clinical mastiffs in the section of PI 4～8 with relative molecular weight of 15 000—75 000 kD，while there were(1224-4)protein spots in those normal whey．There were 31 protein spots different expressions between two groups．In the whey of clinical mastitis，7 protein spots were up—regulated and 6 spots were down-regulated significantly，4 protein spots werent expressed and 1 0 spots were present newly． Key words：mastitis；whey；two—dimensional gel electrophoresis；proteome map 乳房炎是奶牛最常见的一种疾病，它是由各种 业影响最严重、花费最高的病，严重阻碍著奶牛养殖 病因引起的乳房炎症，主要特点是乳汁发生理化性 业和乳品工业的发展．近年来，研究表明，患乳房炎 质及细菌学变化，乳腺