光动力学疗法影响变形链球菌生长和形态的体外研究-第三军医大学学报.DOC

光动力学疗法影响变形链球菌生长和形态的体外研究 许小辉1，蒋 琳1，林居红1，刘明方2（4011471重庆，重庆医科大学附属口腔医院儿童牙病中心11；400016重庆，重庆医科大学实验教学中心2） [摘要] 目的 研究甲苯胺蓝为光敏剂介导的光动力学疗法对变形链球菌体外生长及表面形态的影响，探讨光动力学疗法影响致龋菌生长代谢的作用机制。方法 在不同光和不同光敏剂浓度下通过体外测定光动力学疗法对变形链球菌的生长抑制作用，扫描电镜观察光动力学疗法处理前后菌体形态的改变， 光动力学疗法能显著抑制变形链球菌的体外生长，实验组与对照组相比有显著性差异（P0.05）光敏剂浓度和光剂量存在交互作用（F=19.47, P0.01）R780.1 [文献标志码] A In vitro study of Photodynamic Therapy on the Viability and Morphology of Streptococcus mutans Xu Xiaohui 1, Lin Juhong1, Liu Mingfang2, Jiang Lin (1Children’s dental center , the Affiliated Stomatology Hospital of Chongqing Medical University, Chongqing,401147; 2Teaching center of Chongqing Medical University, Chongqing,400015, China) [Abstract] Objective To evaluate the impact of photodynamic therapy on the viability and morphology of Streptococcus mutans in a planktonic culture. Methods Inhibitory action of toluidine blue O-mediated photodynamic therapy on Streptococcus mutans were analyzed by colony-counting method under different light intensity in vitro conditions;Cell morphologic changes of streptococcus mutans was observed under scanning electron microscope. Samples were prepared and divided into control group , photosensitizer control group, light control group and PDT group. Results There was significant statistics difference (P0.05) between experimental group and control group by PDT. Photosensitizer concentration and the light dosage existenced correlation actions（F=19.47, P0.01）; The bacterial cell surfaces of PDT group became rough , with blister shaped bulge. Conclusion It was demonstrated that PDT was efficient at killing microorganisms and changing bacteria’s morphology. [Key words] Photodynamic therapy; Streptococcus mutans; Dental caries Supported by the project of Chongqing Bureau of Public Health（2008-2-243). Corresponding author:Lin Juhong, Tel:（0238301, E-mail : juhonglin@(photodynamic therapy，PDT)近年来，光动力学疗法逐渐应用于领域。变形链球菌是公认的主要致龋菌。本实验通过体外在不同光和不同光敏剂浓度下光动力学疗法对变形链球菌的生长抑制作用，并用扫描电镜观察光动力学疗法处理前后变形链球菌菌体形态的改变，初步探讨光动力学