细胞医学.doc

XP-180 研究论文： XP-180 对人类癌细胞抗癌实验室研究 CHUNG HIN CHUI1,2, ROBERTO GAMBARI3, FUNG YI LAU2, GREGORY YIN MING CHENG2, RAYMOND SIU MING WONG2, STANTON HON LUNG KOK1, JOHNNY CHEUK ON TANG1, IVY TUANG NGO TEO1,2, FILLY CHEUNG1,2, CHOR HING CHENG1,2, KWOK PING HO1,2, ALBERT SUN CHI CHAN1 and ALFONSO WONG4 1Anti-Cancer Research Center, Central Laboratory of the Institute of Molecular Technology for Drug Discovery and Synthesis, State Key Laboratory of Chinese Medicine and Molecular Pharmacology, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University; 2Department of Medicine and Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, P.R. China; 3ER-GenTech, Department of Biochemistry and Molecular Biology, Section of Molecular Biology, University of Ferrara, Italy; 4International Naturopathic Cancer Research Institute, P.R. China. Abbreviated running title: XP-180 on cancer cells in vitro Key words: apoptosis, neoplasms, Oncogen XP-180 Correspondence to: Dr. Tang Cheuk On, Johnny. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Hong Kong, China. XP-180论文摘要 本论文研究益菌提炼药液「御元素XP-180」的抗癌增生及杀癌效果。研究的癌瘤细胞包括三类人类肿瘤细胞及一类非肿瘤类急性血癌细胞(Acute myelogenous leukemia) KG1a。三类肿瘤细胞分别为非小细胞肺癌细胞(non-small cell lung cancer) A549，乳癌细胞(Breast cancer)MDA-MB231及肝腺脏癌细胞(Liver adenocarcinoma) SK-Hep1。「御元素XP-180」从研究中证实对这四类癌种有非常高效的杀癌抗癌效果。 研究方法为「御元素」经稀释至10%（即稀释为十份之一）后，加进各类癌细胞种碟。经48小时癌细胞孵养，发现「御元素」有惊人的击杀癌细胞功能(Cytotoxic activity)。在细胞形状上(morphology)亦观察到癌细胞经10%「御元素」接触後出现细胞受破坏後收缩形态，并且癌细胞的黏附性亦失去功效，继而失去癌细胞基本功能。 “同本生物生殖被固定方法”(Anchorage dependent clonogenicity assay)测试肿瘤类癌细胞及 “半固体甲基细胞膜质丛测试”(Semi-solid methylcellulose colony formation assay)血癌细胞同时指出「御元素XP-180」有极强力阻止癌细胞复发功能。 当使用血癌细胞KG1a为示范模式，「御元素XP-180」能刺激Caspase 3, 8, 9细胞酵素功能，并且能在接触多类癌细胞的2小时内，令癌细胞的腺粒体膜受破坏而导致癌细胞死亡。 总括来说，实验研究指出「御元素XP-180」是非常有效的抗癌药物。 序言 Phaffia rhodozyma (Xanthophyllomyces denrorhous) 萃取物能活跃的抑制数种癌细胞增生和促使癌细胞死亡（8-10）。这两种萃取物拥有强大的抗氧化作用。[御元素XP-180]是从超过1