检测血清抗核小体抗体水平在SLE诊断及转归中的研.docVIP

　　检测血清抗核小体抗体水平在SLE诊断及转归中的研 【关键词】 抗核小体抗体；红斑狼疮；系统性；皮肌炎 ［摘 要］ 目的:研究血清抗核小体抗体(AnuA)水平监测在系统性红斑狼疮(SLE)诊断中的价值。方法：采用酶联免疫吸附法（ELISA）测定62例SLE，52例亚急性皮肤型红斑狼疮（SCLE），10例皮肌炎和30例健康人血清AnuA水平，采用SLE疾病活动指数0.05)。AnuA水平与SLEDAI有明显相关性（P=0.015,r=0.441），SLE患者治疗后血清AnuA水平与治疗前相比明显降低（Plt;0.05）。结论：AnuA水平高低对诊断SLE、监测病情活动和判断疗效有意义。 　　［关键词］ 抗核小体抗体；红斑狼疮；系统性；皮肌炎 　　Examine the Level of Antinucleosome Antibody in Blood Serum in the Diagnostics of Systemic Lupus Erythematosus and The Research ine the level of Antinucleosome antibody (AnuA) in blood serum to discover the value in the diagnostics of Systemic Lupus Erythematosus (SLE) and pare the results from before and after treatments respectively.Methods Enzymelinked immunosorbant assay (ELISA) from 62 samples of SLE,52 samples of Subacute Cutaneous Lupus Erythematosus (SCLE),10 samples of Dermatomyositis and 30 healthy samples,respectively.In order to determine and grade the criterion of state of illness,the Systemic Lupus Erythematosus Activity Index (SLEDAI) has been adopted.Results The levels of AnuA in serum from the SLE samples the samples of atomyositis patients and healthy specimens (Plt;0.01).The levels of AnuA in serum from the active SLE samples,that are much higher than that from the samples atomyositis samples and the healthy ones (Plt;0.05).The difference bet dermatomyositis and healthy samples has notgot any statistic significance (Pgt;0.05).There is apparent pertinence bet remarkably reduced ents had been given to SLE patients paring ents (Plt;0.05).Conclusion The levels of AnuA is valid to help to diagnose the SLE,monitor the disease and estimate the curative effect. 　　Key e antibody；Lupus Erythematosus；Dermatomyositis；Systemic 系统性红斑狼疮（SLE）是一种临床比较常见的自身免疫性结缔组织病。核小体是染色质的基本单位，在细胞凋亡过程中，核酸内酶活化后切割核染色质释放出核小体，核小体是靶抗原，诱导抗核小体抗体（AnuA）的产生［1］，通过研究发现，SLE患者体内存在大量的抗核小体抗体，我们采用酶联免疫吸附实验（ELISA）法检测SLE、SCLE、皮肌炎和健康人血清中AnuA水平的变化，了解和研究AnuA在SLE诊断中的作用及与病情活动性的关系和治疗前后的变化。 　　1 资料 　　1998年1月至2005年6月我院门诊及住院病人确诊SLE、SCLE患者114例，女107例，男7例，年龄11岁~70岁，平均年龄31岁；病程30 d~9.5 a，平均2