大蒜素诱导人胃癌sgc-7901细胞凋亡 - 中国细胞生物学学报.pdfVIP

中国细胞生物学学报 Chinese Journal of Cell Biology 2017, 39(1): 6– 12 DOI: 10.11844/cj cb.2017.01.02 19 研究论文 大蒜素诱导人胃癌SGC-7901细胞凋亡 * 朱少伟 邵淑丽 张 蕾 张伟伟 陈 琳 刘 畅 (齐齐哈尔大学生命科学与农林学院, 齐齐哈尔161000) 摘要 该研究探讨了大蒜素诱导人胃癌SGC-7901细胞凋亡及其作用机制。用不同浓度大蒜 素作用人胃癌SGC-7901细胞48 h 。通过MTT法检测细胞活性。光学、激光共聚焦显微镜下观察细 胞形态变化。流式细胞术检测细胞凋亡率和细胞周期。qRT-PCR和Western blot检测Bax 、Bcl-2基 因和蛋白的表达水平。结果显示, 大蒜素处理细胞48 h 的IC50值为78 μg/mL, 显微镜下可观察到明 显的凋亡现象, 细胞凋亡率为(61.15±3.77)%, 细胞阻滞于G1期, Bcl-2基因和蛋白表达均下降, Bax基 因和蛋白表达均增加(P0.05) 。综上所述, 在一定浓度范围内, 大蒜素能抑制人胃癌SGC-7901细胞 Bax Bcl-2 增殖, 诱导细胞凋亡, 呈剂量依赖性, 并可上调 基因表达, 下调 基因表达。 关键词 SGC-7901细胞; 大蒜素; 凋亡; Bax; Bcl-2 The Induction of Apoptosis in Human Gastric Cancer SGC-7901 Cells by Allicin Zhu Shaowei, Shao Shuli*, Zhang Lei, Zhang Weiwei, Chen Lin, Liu Chang (College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161000, China) Abstract This work was aimed to investigate the apoptosis-inducing effect of allicin in human gastric cancer SGC-7901 cells and analyze its molecular mechanisms. MTT assay was used to determine SGC-7901 cells activity. Morphological changes of cells were observed under optical and laser confocal microscope. Apoptosis rate and cell cycle distribution were detected by flow cytometry. The expressions of Bax and Bcl-2 were measured by qRT-PCR and Western blot. The results showed that the IC50 was 78 μg/mL after the SGC-7901 cells treated with allicin for 48 h in this study. The cells treated with allicin showed a typical apoptotic morphology. The apoptosis rate was up to (61.15±3.77)% and the G1 arrest was induced significantly after treated with allicin. The results sug