微生物法测定多种维生素片中的生物素含量 - 汤臣倍健.pdf

6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug. , 2015 * 黄进丽 , 杨祖伟 (, 519040) 摘 要: 目的 方法 GB 5413.19-2010 , MRS , 65 ℃ ~70 ℃, 630 nm , , 结果 0.01~0.1 ng/mL , r20.999, 80%100%120%, 99.8%96.1%97.2%结论 , 关键词: ; ; Microorganism determination of biotin content in multi-vitamins tablets * HUANG Jin-Li , YANG Zu-Wei (By-Health Co ., Ltd., Zhuhai 519040, China) ABSTRACT: Objective To determine the content of biotin in multi-vitamins tablets by microbial assay. Methods On the basis of GB 5413.19 2010 analysis method, bacteria suspension were prepared by lactic acid bacteria culture medium to (MRS) broth culture medium, the sample was extracted by water bath ultrasonic after 65 ℃ ~70 ℃ ; then culture medium absorbance was measured by absorbance enzyme standard instrument under the 630 nm, and to quantitatively detect the content of biotin. The standard curve, precision and accuracy of contrast test were compared with the national standard method, and the new method had carried on the standard addition recovery test. Results Biotin concentration within the