窄冠白杨组培快繁与再生体系的建立.pdfVIP

Chinese Agricultural Science Bulletin Vo1．24 No．7 2008 July http：llwww．casb．org．cn ·60· 窄冠白杨组培快繁与再生体 系的建立 李际红，邢世岩，焦 振，刘国兴，张友朋 (山东农业大学林学院，泰安271018) 摘 要：以窄冠白杨为试材，对其组培快繁与再生体系的建立进行了初步的研究。研究表明．抗氧化剂聚 乙烯吡咯烷酮(PVP)和抗坏血酸(Vc)能有效降低外植体的褐化；该品种外植体增殖的最适培养基为 1／2MS+BA0．3mg／L+NAA0 05mg／L，平均分化率为87％，增殖系数达7．5倍，与其它处理组合均呈极显著 差异。硝普钠(SNP)12mg／L极显著地促进叶片不定芽分化，其再生频率与再生芽个数分别达到96．5％与 8．2。窄冠白杨适宜的生根培养基为I／2MS+IBA0．3mg／L。 关键词：窄冠白杨；组培快繁；再生体系：硝普钠 中图分类号：$722．37 文献标识码：A Tissue Culture and Regeneration System from Leaves of Populus Leucopyramidal~in vitro Li Jihong，Xing Shiyan，Jiao Zhen，Liu Guoxing，Zhang Youpeng (College of Forest Shandong Agricttlture University，Tai’an 271018) Abstract：In this report，tissue culture and regeneration system from leaves in vitro have been extensively in— vestigated utilizing Populus Leucopyramidalis as experiment materials．The results showed that the anti-oxi- dation materials po1yviny1pyrr0nd0ne(PVP)and ascorbic acid(Vc)decreased browning efficiently；the best medium for proliferation was 1／2 MS+BA0．3mg／L+NAA O．05mg／L with differentiation percentage of 87％ and proliferation multiples of 7．5 respectively，and there was a great significant difference compared with oth- er treatments．The differentiation and regeneration of adventitious shoot from leaves increased significantly in the medium MS containing BA0．3mg／L and NAAO．05mg／L supplemented with 12mg／L sodium nitroprusside (SN P)in which regeneration frequency and average number of shoots were 100％and 12 respectively．The best rooting medium was 1／2 MS+IBA0．3mg／L． Key words：populus leucopyramidalis，tissue culture，regeneration system，sodium nitroprusside 窄冠白杨 1号、3号、6号 (populus leuc