栀子高频植株再生体系相关因子的优化.pdfVIP

湖南农业科学 2008，(3)：17～20 Hunan A cultural Sciences 栀子高频植株再生体系相关因子的优化 尹明华，洪森荣 (上饶师范学院生命科学系，江西上饶 334001) 摘 要：采用正交实验和单因子实验以及植物组织培养的方法，对栀子高频植株再生体系有关因子的进一步优化进行了探讨。结果 表明，栀子带芽茎段再生体系的最佳消毒方式为70％酒精处理30 S后再用0．1％HgCl：处理 15 min；栀子带芽茎段再生的最佳培养 基为MS+6一BA 2 ms／L+NAA 0．5 mg／L；光照培养有利于栀子带芽茎段的再生；栀子带芽茎段再生的最佳蔗糖浓度为25 s／L，最佳琼 脂浓度为7．5 gL，最佳pH值为6，最佳培养温度为25℃。 关键词：栀子；高频植株；带芽茎段渖 生 中图分类号：$687 9 文献标识码：A 文章编号：1006—060X(2008)03—0017—04 Optimization of Correlation Factors of High Efficiency Regeneration System of Gardenia Jasminoides Ellis． YIN Ming-hua，HONG Sen-rong (L／fe Science Department ofShangrao Normal College，Shangrao 334001，PR cj Abstract：Objective：to illustrate the effects of several factors on dedifferentiation and redifferentiation of Gardenia asmi— noides Ellis．earthnut．Method：orthogonal experiment，single factor experiment and tissue culture were applied．Result and Conclusion：the best disinfection way of Gardenia jasminoides Ellis．stems with a bud was firstly using 70％ethyl alcohol to process for 30 minutes then using 0．I％HgC12 to process for 15 seconds again．The best regeneration culture medium of Gnr— den／a jasminoides Ellis stems with a bud was MS+6-BA 2 ms／L+NAA 0．5 ms／L．The light culture was more advantageous to Gardenia jasminoides Ellis．stem regeneration．For regeneration system of Gardenia jasminoides Ellis．stems with a bud， the best concentration sucrose and agar was 25 g／L and 7．5 s／L respectively，the best pH value was 6，the best culture tern— preture was 25~C． Key words：Gardeniajasminoides Ellis．；high frequency plantlet；stem with a bud；regeneration 栀子(Gardenia asminoides Ellis．)又名黄栀子、 道，且大多集中于桅子培养中的激素选配及其增殖 山枝子、山桅、林兰等，为茜草科栀子属植物，主产 发根等方面 。为了提高繁殖系数，在短期内大量 于湖南、江西、福建、浙江、四川、湖北等地[1l。栀子是