抗虫绿豆对绿豆象生长发育及体内几种酶活性的影响.PDFVIP

植物保护学报Journal of Plant Protection ，2017 ，44 （3）：420-426 DOI ：10.13802/ki.zwbhxb.2017.2016095 抗虫绿豆对绿豆象生长发育及体内几种 酶活性的影响 1 2 1* 成小芳 张耀文 张仙红 （1. 山西农业大学农学院，太谷030801 ；2. 山西省农业科学院作物科学研究所，太原030000 ） 摘要：为明确抗虫绿豆对绿豆象Callosobruchus chinensis L. 生长发育的抑制作用，采用室内人工接 虫和生化测定的方法，研究了绿豆象幼虫取食抗虫绿豆后其体重、体内消化酶、保护酶和解毒酶活 性及成虫羽化率和体重的变化。结果表明，取食抗虫绿豆的绿豆象幼虫体重、雌、雄成虫体重以及 成虫羽化率分别是取食感虫绿豆的0.69、0.69、0.71 及0.14倍；取食抗虫绿豆8~ 16 d 的绿豆象幼虫 中肠总蛋白酶、α-淀粉酶和α-葡萄糖苷酶活性均显著低于对照；保护酶超氧化物歧化酶、过氧化物 酶和过氧化氢酶与解毒酶羧酸酯酶和谷胱甘肽转移酶的活性在绿豆象幼虫取食8~ 12 d 时均显著 高于对照，但取食14~ 16 d 时，2 种解毒酶活性与对照差异不显著。表明抗虫绿豆可抑制绿豆象幼 虫中肠消化酶活性，影响其体内保护酶和解毒酶活性，从而使绿豆象幼虫不能正常生长发育。 关键词：绿豆象；抗虫绿豆；消化酶；保护酶；解毒酶 Effects of insect-resistant mung bean on development and activities of several enzymes of Chinese bean weevil Callosobruchus chinensis L. (Coleoptera: Bruchidae) Cheng Xiaofang1 Zhang Yaowen2 Zhang Xianhong1* （1. College of Agronomy, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China; 2. Institute Crop Research, Shanxi Academy of Agricultural Sciences, Taiyuan 030000, Shanxi Province, China） Abstract: To clarify the inhibitory effect of insect-resistant mung bean on growth and development of Chinese bean weevil Callosobruchus chinensis L., the body weight and activities of enzymes for diges- tion, protection, and detoxification of larvae treated with insect-resistant mung bean, and the eclosion rate and weight of imagoes were detected after artificial inoculation in the laboratory. The results showed that the larval weight, the adult eclosion rate, and the body weight of the male and female adults fed on insect-resistant mung bean were 0.69, 0.14, 0.69 and 0.71 times as those on the