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肾上腺髓质素在大鼠延髓头端腹外侧区对压力反射敏感神经元的作用.pdf
Acta Physiologica Sinica , June 25, 2006, 58 (3): 193-200 193
Research Paper
Effect of adrenomedullin on the activity of barosensitive neurons in the
rostral ventrolateral medulla of rats
FAN Ming-Xin, LI Xia, WANG Jin, CAO Yin-Xiang, SHEN Lin-Lin, ZHU Da-Nian*
Department of Physiology and Pathophysiology, Shanghai Medical College of Fudan University, Shanghai 200032, China
Abstract: To investigate the eletrophysiological effect of rat adrenomedullin (rADM) on barosensitive neurons in the rostral ventrolat-
eral medulla (rVLM) and its potential mechanisms, the extracellular recording and multi-barrel iontophoresis methods were used. Of the
29 barosensitive neurons in the rVLM, 20 neurons demonstrated excitatory response to iontophoretically applied rADM and increased
the firing rate from (10.8 ± 2.7) spikes/s to (14.6 ± 3.6), (19.8 ± 4.7) and (31.9 ± 6.4) spikes/s (P0.05, n=20) at the current of 30, 60
and 90 nA, respectively. Application of human adrenomedullin (22-52) [hADM (22-52)], a specific antagonist of rADM receptor,
distinctly attenuated the augmentation of firing rate induced by rADM—the firing rate was increased by 15.4% [(11.4 ± 2.5) spikes/
s, P0.05, n=10]. Another antagonist, human calcitonin gene-related peptide (8-37) [hCGRP (8-37)] had no significant effect on
rADM-induced excitation. Other 23 barosensitive neurons were recorded to test the influence of nitric oxide synthase (NOS) inhibitors
on the excitatory effect of rADM. In 10 neurons, 7-NiNa (neuronal NOS inhibitor) decreased the firing rate from (10.1 ± 3.5) spikes/s
to (7.5 ± 2.5), (5.3 ± 2.1) and (3.1 ± 1.4) spikes/s (P0.05, n=10) at the current of 10, 20 and 40 nA, respectively. The excitatory effect
of rADM (60 nA, 30 s) during 7-NiNa application was nearly eliminated and the magnitude of firing rate was increased only by 17%
of the basal level (6.2 ± 1.9) spikes/s (P0.05, n=7). While aminoguanidine (AG
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