海马体分离提取方法.docxVIP

This is a dorsal view of a mouse?s skull, in order to access the brain you can cut along the coronal suture and sagittal suture then pull off both sides of parietal bone and interparietal boneProcedures:Now the skull is open and you can see the brain clearly, in order to get the hippocampus you?d better keep the brain in the cranial cavity so the brain will not move when you continue with the following stepsProcedures:In order to expose the hippocampus you need to remove the cerebral cortex covering it. The first incision is at the end of the hemisphere; the incision should be about 0.7mm deep for most adult mouse that you might not hurt the hippocampus while to expose it.The 2nd incision is about 1.5-2mm in front of the first one, this incision you need cut into the lateral ventricle, both of the incisions go to the ventral of the brain and meet there. Now this piece of cortex is free, pull it up, you will see the hippocampus just like in this picture, also you can see the CSF in the opened ventricle.?Procedures:Keep working on the other side of the brain pull up both sides of the cortex that covering the hippocampus along the ventricle. Now you can see the dorsal part of the hippocampus. Separate the rest of the hippocampus from the cortex covering it along the surface of the hippocampus towards the ventral part of the hippocampus.Procedures:Keep working on the other side of the brain pull up both sides of the cortex that covering the hippocampus along the ventricle. Now you can see the dorsal part of the hippocampus. Separate the rest of the hippocampus from the cortex covering it along the surface of the hippocampus towards the ventral part of the hippocampus.Procedures:Now you need to free the hippocampus from the surrounding tissue.Procedures:Here is the hippocampus picked out from the brain.Procedures:The rest of the brain that hippocampus has been taken off.