沙田柚谷胱甘肽转移酶基因CmGST的生物信息学及表达分析.pdfVIP

Botanical Research 植物学研究, 2017, 6(2), 47-54 Published Online March 2017 in Hans. /journal/br /10.12677/br.2017.62008 Bioinformatics and Expression Analysis of GST Gene Encoding Glutathione S-Transferase in Citrus maxima cv. Shatian Yu Qiong Luo, Jun Xiao, Shaomei Li, Xiaoxia Long, Huaying Liu, Xinmin Qin* College of Life Science, Guangxi Normal University, Guilin Guangxi th th th Received: Mar. 7 , 2017; accepted: Mar. 25 , 2017; published: Mar. 28 , 2017 Abstract In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus maxima cv. Shatian Yu were sequences by high-throughput sequencing technology. The gluta- thione S-transferase gene (CmGST) sequence of Citrus maxima cv. Shatian Yu was obtained through differential analysis method. The fu11 length of CmGST gene was 872 bp (GenBank accession number: KY612456), and the ORF (Open Reading Frame) length was 657 bp, encoding a protein polypeptide of 219 amino acids with a predicted molecular weight of 25.00 kDa and pI of 5.48. Bioinformatics analysis showed that the CmGST protein contained the Tau GST-specific N-terminal domain (G site) and the C-terminal domain (H site). The homology analysis of amino acid sequence indicated that the glutathione S-transferase protein shared high homology with glutathione S- transferase protein of Citrus clementina (98%). This work provides a systemic sequence analysis of the glutathione S-transferase protein of Citrus maxima cv. Shatian Yu. It will provide the useful reference for further investigation of its structure and function. Keywords Citrus maxima cv. Shatian Yu, Glutathione S-Transferase