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沙田柚谷胱甘肽转移酶基因CmGST的生物信息学及表达分析
Botanical Research 植物学研究, 2017, 6(2), 47-54
Published Online March 2017 in Hans. /journal/br
/10.12677/br.2017.62008
Bioinformatics and Expression Analysis of
GST Gene Encoding Glutathione
S-Transferase in Citrus maxima cv.
Shatian Yu
Qiong Luo, Jun Xiao, Shaomei Li, Xiaoxia Long, Huaying Liu, Xinmin Qin*
College of Life Science, Guangxi Normal University, Guilin Guangxi
th th th
Received: Mar. 7 , 2017; accepted: Mar. 25 , 2017; published: Mar. 28 , 2017
Abstract
In this paper, the transcriptome of the self-pollinated style and cross-pollinated style of Citrus
maxima cv. Shatian Yu were sequences by high-throughput sequencing technology. The gluta-
thione S-transferase gene (CmGST) sequence of Citrus maxima cv. Shatian Yu was obtained through
differential analysis method. The fu11 length of CmGST gene was 872 bp (GenBank accession
number: KY612456), and the ORF (Open Reading Frame) length was 657 bp, encoding a protein
polypeptide of 219 amino acids with a predicted molecular weight of 25.00 kDa and pI of 5.48.
Bioinformatics analysis showed that the CmGST protein contained the Tau GST-specific N-terminal
domain (G site) and the C-terminal domain (H site). The homology analysis of amino acid sequence
indicated that the glutathione S-transferase protein shared high homology with glutathione S-
transferase protein of Citrus clementina (98%). This work provides a systemic sequence analysis
of the glutathione S-transferase protein of Citrus maxima cv. Shatian Yu. It will provide the useful
reference for further investigation of its structure and function.
Keywords
Citrus maxima cv. Shatian Yu, Glutathione S-Transferase
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