植物油脂生物合成表面活性剂及产物分析.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.4 植物油脂生物合成表面活性剂及产物分析 李静，邓毛程，陈维新，王瑶，陈赞荣 （广东轻工职业技术学院，广东广州 510300） 摘要：为了利用废弃油脂制备生物表面活性剂，用嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)WO-S14 对不同植物油脂进 行发酵，并对产物进行系统分析。通过发酵试验与性能分析，发现芝麻油的发酵产物具有优良的表面性能，对煤油的乳化率 E24 为 75.22%，使水的表面张力降低至27.90 mN/m，且在较广的环境条件范围内具有良好稳定性。采用柱层析方法获得分离纯化的表面活 性剂产物，通过薄层层析（TLC ）、红外光谱（IR ）、气质联用(GC-MS)、电喷雾质谱(ESI-MS)和串联质谱(MS/MS)等手段分析了产物 的化学组成和结构，结果表明以芝麻油生物合成的表面活性剂主要是一种具有环状结构的脂肽，分子量为 814，由含有 15 个碳原子 的β-羟基脂肪酸和含有 6 个氨基酸分子的短肽组成，肽链中氨基酸连接顺序为：-Met-Glu-Ala-Ser-Ala-Val-，是一种新型脂肽类生物表 面活性剂。 关键词：生物合成；产物分析；发酵；植物油脂；生物表面活性剂；脂肽 文章篇号：1673-9078(2016)4-171-176 DOI: 10.13982/j.mfst.1673-9078.2016.4.028 Biosynthesis and Characterization of Novel Surfactants Prepared using Vegetable Oils LI Jing, DENG Mao-cheng, CHEN Wei-xin, WANG Yao, CHEN Zan-rong (Guangdong Industry Polytechnic, Guangzhou 510300, China) Abstract: Different vegetable oils were fermented using Stenotrophomonas maltophilia WO-S14, with the objective of preparing biosurfactants from waste oil. The properties of the fermentation products were analyzed thoroughly and systematically. The fermentation product of sesame oil showed good surface properties; its emulsification index (E24) against kerosene was 75.22%; it could reduce the surface tension of water to 27.90 mN/m; it was highly stable under a wide range of environmental conditions. The surfactant product was purified by column chromatography and the chemical composition and structure of the purified product were studied by thin layer chroma